Project Details
Description / Abstract
The perpetual motion of the Earth on its axis and the orbit around the sun generates a rhythmic environment for life on Earth. Organisms of all types have responded by evolving biological clocks and calendars that allow anticipation of day and night, and the changing seasons. Recent research into this 'chronobiology' has characterised a small number of genes, called clock genes, which generate the intrinsic daily rhythm. These genes show conserved features in their structure and function from insects to man, reflecting their ancient evolution. Clock genes act within most cells of the body to produce a near 24-hour rhythmic output, and cells communicate to regulate daily rhythms in sleep-activity, feeding, hormone secretion and many other characteristics. Much less is understood about the molecular basis of longer-term timers and the mechanism by which animals respond to changing day length to synchronise seasonal rhythms. In mammals, a key aspect of seasonal timekeeping is the production of melatonin by the pineal gland. Melatonin is produced only at night and the pattern directly reflects the length of the night, and it is the changes in this hormonal signal that conveys information about time of year around the body. Long daily bouts of melatonin act as the signal for winter and short bouts for summer. Our previous work has shown that the changing pattern of melatonin is decoded through the switching on and off of specific clock genes. Notably, the melatonin increase at dusk activates Cry1 gene expression, and the melatonin decline at dawn activates Per1 gene expression. Based on this we have proposed an 'internal coincidence hypothesis' for photoperiod time measurement. This states that the extent of interaction between CRY1 and PER1 proteins, which is dictated by the period from dusk to dawn - the melatonin signal, governs the seasonal response. The daily clock mechanism has thus been co-opted for seasonal timing in melatonin-responsive cells that are located in the brain and pituitary gland. Now we plan to test this hypothesis using transgenic sheep in which the Cry1 gene is selectively neutralised. This strategy depends on our recent demonstration that modified DNA sequences can be efficiently introduced into sheep embryos and are expressed in lambs. The sheep is used as a model because of its very well characterised seasonal biology. The aim is to use two different 'transgenes' to interfere with endogenous expression of the Cry1 gene. The prediction is that this will not affect normal development, but will block photoperiodic responsiveness. The biology of this 'sheep for all seasons' will be of major interest to chronobiologists, and the use of transgenesis in sheep opens a new era in the study of genetic control in a long-lived species.
Status | Finished |
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Effective start/end date | 1/03/07 → 30/06/11 |
Links | https://gtr.ukri.org:443/projects?ref=BB%2FE017193%2F1 |