A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Thabat Khatib; Berndt Marino Müller; Peter McCaffery

doi:10.1007/978-1-0716-2453-1_15

A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Thabat Khatib^* (Corresponding Author)
, Berndt Marino Müller
, Peter McCaffery

^*Corresponding author for this work

University of Aberdeen

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Abstract

The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5′ untranslated region (5′ UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.

Original language	English
Title of host publication	Bioluminescence
Subtitle of host publication	Methods and Protocols, Volume 1
Editors	Sung-Bae Kim
Place of Publication	New York
Publisher	Humana Press
Pages	197-207
Number of pages	11
Volume	1
Edition	4
ISBN (Electronic)	978-1-0716-2453-1
ISBN (Print)	978-1-0716-2452-4, 978-1-0716-2455-5
DOIs	https://doi.org/10.1007/978-1-0716-2453-1_15
Publication status	Published - 14 Jul 2022

Publication series

Name	Methods in Molecular Biology
Publisher	Humana Press
Volume	2524
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

GluA1
Gria1
GluR1
Retinoid
Non-genomic
Cytoplasm
Synaptic scaling

Access to Document

10.1007/978-1-0716-2453-1_15Licence: Unspecified

Cite this

Khatib, T., Müller, B. M., & McCaffery, P. (2022). A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. In S.-B. Kim (Ed.), Bioluminescence: Methods and Protocols, Volume 1 (4 ed., Vol. 1, pp. 197-207). (Methods in Molecular Biology; Vol. 2524). Humana Press. https://doi.org/10.1007/978-1-0716-2453-1_15

A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. / Khatib, Thabat (Corresponding Author); Müller, Berndt Marino ; McCaffery, Peter.
Bioluminescence: Methods and Protocols, Volume 1. ed. / Sung-Bae Kim. Vol. 1 4. ed. New York: Humana Press, 2022. p. 197-207 (Methods in Molecular Biology; Vol. 2524).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

Khatib, T, Müller, BM & McCaffery, P 2022, A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. in S-B Kim (ed.), Bioluminescence: Methods and Protocols, Volume 1. 4 edn, vol. 1, Methods in Molecular Biology, vol. 2524, Humana Press, New York, pp. 197-207. https://doi.org/10.1007/978-1-0716-2453-1_15

Khatib T, Müller BM , McCaffery P. A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor. In Kim SB, editor, Bioluminescence: Methods and Protocols, Volume 1. 4 ed. Vol. 1. New York: Humana Press. 2022. p. 197-207. (Methods in Molecular Biology). Epub 2022 Jul 13. doi: 10.1007/978-1-0716-2453-1_15

@inbook{119b8349aac943f29e1d1666b388cf0f,

title = "A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor",

abstract = "The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5′ untranslated region (5′ UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.",

keywords = "GluA1, Gria1, GluR1, Retinoid, Non-genomic, Cytoplasm, Synaptic scaling",

author = "Thabat Khatib and M{\"u}ller, \{Berndt Marino\} and Peter McCaffery",

year = "2022",

month = jul,

day = "14",

doi = "10.1007/978-1-0716-2453-1\_15",

language = "English",

isbn = "978-1-0716-2452-4",

volume = "1",

series = "Methods in Molecular Biology",

publisher = "Humana Press",

pages = "197--207",

editor = "Sung-Bae Kim",

booktitle = "Bioluminescence",

address = "United States",

edition = "4",

}

TY - CHAP

T1 - A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

AU - Khatib, Thabat

AU - Müller, Berndt Marino

AU - McCaffery, Peter

PY - 2022/7/14

Y1 - 2022/7/14

N2 - The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5′ untranslated region (5′ UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.

AB - The present protocol describes a bioluminescence reporter assay developed to quantify the ability of synthetic agonists of retinoic acid receptors (RARs) to activate glutamate receptor subunit 1 (GluR1) translation. The reporter assay uses firefly luciferase under the control of the GluR1 5′ untranslated region (5′ UTR) which is bound by RARs to regulate its translation. This method is used to demonstrate the role of RARα in retinoic acid regulation of GluR1 translation. This method may also be used to screen drugs that influence RAR induction of GluR1 translation as an important mechanism controlling learning and memory in the brain.

KW - GluA1

KW - Gria1

KW - GluR1

KW - Retinoid

KW - Non-genomic

KW - Cytoplasm

KW - Synaptic scaling

U2 - 10.1007/978-1-0716-2453-1_15

DO - 10.1007/978-1-0716-2453-1_15

M3 - Chapter (peer-reviewed)

SN - 978-1-0716-2452-4

SN - 978-1-0716-2455-5

VL - 1

T3 - Methods in Molecular Biology

SP - 197

EP - 207

BT - Bioluminescence

A2 - Kim, Sung-Bae

PB - Humana Press

CY - New York

ER -

A Bioluminescence Reporter Assay for Retinoic Acid Control of Translation of the GluR1 Subunit of the AMPA Glutamate Receptor

Abstract

Publication series

Keywords

Access to Document

Fingerprint

Cite this