A facile analytical method for reliable selectivity examination in cofactor NADH regeneration

Tony Saba; Joseph W. H. Burnett; Jianwei Li; Panagiotis Kechagiopoulos; Xiaodong Wang

doi:10.1039/c9cc07805c

A facile analytical method for reliable selectivity examination in cofactor NADH regeneration

Tony Saba
, Joseph W. H. Burnett
, Jianwei Li
, Panagiotis Kechagiopoulos
, Xiaodong Wang^*

^*Corresponding author for this work

Lancaster University

Research output: Contribution to journal › Article › peer-review

79 Citations (Scopus)

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Abstract

This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.

Original language	English
Pages (from-to)	1231-1234
Number of pages	4
Journal	Chemical Communications
Volume	56
Issue number	8
Early online date	18 Dec 2019
DOIs	https://doi.org/10.1039/c9cc07805c
Publication status	Published - 25 Jan 2020

Bibliographical note

This work was supported by The Royal Society (ICA\R1\180317 and IES\R3\170162).

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10.1039/c9cc07805cLicence: CC BY

Saba_et_al_ChemComm_AFacileAnalytical_VoR
© The Royal Society of Chemistry 2020. This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. https://creativecommons.org/licenses/by/3.0/
Final published version, 2.51 MBLicence: CC BY

Cite this

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abstract = "This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.",

author = "Tony Saba and Burnett, \{Joseph W. H.\} and Jianwei Li and Panagiotis Kechagiopoulos and Xiaodong Wang",

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AU - Saba, Tony

AU - Burnett, Joseph W. H.

AU - Li, Jianwei

AU - Kechagiopoulos, Panagiotis

AU - Wang, Xiaodong

N1 - This work was supported by The Royal Society (ICA\R1\180317 and IES\R3\170162).

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N2 - This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.

AB - This study demonstrates a novel method to quantify selective (1,4-NADH) and unselective products (1,2- and 1,6-NADH) in NADH regeneration using combined UV-Vis spectroscopy and biological assays. The validity of the proposed method was tested in the Pt/C promoted NAD+ hydrogenation using hydrogen as reducing agent.

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SP - 1231

EP - 1234

JO - Chemical Communications

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IS - 8

ER -

A facile analytical method for reliable selectivity examination in cofactor NADH regeneration

Abstract

Bibliographical note

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