Abstract
A rainbow trout interferon (IFN) reporter system has been established by selection of a stable cell line, RTG-P1, transfected with a plasmid expressing the firefly luciferase gene under the control of the promoter for the IFN-induced gene Mx1. After 148 passages, the luciferase expression was still highly induced by polyinosinic:polycytidylic acid (poly I:C) in RTG-P1 cells. Different IFN inducers (dsRNA, viral hemorrhagic septicaemia virus or conditioned medium containing rainbow trout antiviral activity) were able to stimulate the IFN-reporter system in RTG-P1, showing that this cell line can be used to study the activation of the IFN pathway in various contexts. Pyrrolidine dithiocarbamate (PDTC), an NF-kappaB inhibitor, significantly blocked poly I:C induced luciferase accumulation in RTG-P1 at intermediate doses (1-10 muM), suggesting that Mx1 induction through the IFN signalling pathway is NF-kappaB-dependent in fish. This inhibition was not observed for doses of 50 muM or higher. The RTG-P1 reporter system constitutes an interesting tool to study the induction and regulation of IFN signalling in teleost fish. (C) 2004 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 793-801 |
| Number of pages | 9 |
| Journal | Developmental and Comparative Immunology |
| Volume | 28 |
| DOIs | |
| Publication status | Published - 2004 |
Keywords
- NF-KAPPA-B
- DOUBLE-STRANDED-RNA
- HEMORRHAGIC SEPTICEMIA VIRUS
- VESICULAR STOMATITIS-VIRUS
- MOLECULAR-CLONING
- ATLANTIC SALMON
- INFLUENZA-VIRUS
- EXPRESSION
- GENE
- CELLS