Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

H  Monkkonen; P  Moilanen; J  Monkkonen; J C  Frith; M J  Rogers; S  Auriola

Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

H Monkkonen, P Moilanen, J Monkkonen, J C Frith, M J Rogers, S Auriola

Applied Medicine

Research output: Contribution to journal › Article › peer-review

36 Citations (Scopus)

Abstract

Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	395-403
Number of pages	9
Journal	Journal of Chromatography B: Biomedical Sciences and Applications
Volume	738
Publication status	Published - 2000

Keywords

clodronate
MOLD DICTYOSTELIUM-DISCOIDEUM
IN-VITRO
BISPHOSPHONATES
AMEBAS

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Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry. / Monkkonen, H ; Moilanen, P ; Monkkonen, J et al.
In: Journal of Chromatography B: Biomedical Sciences and Applications, Vol. 738, 2000, p. 395-403.

Research output: Contribution to journal › Article › peer-review

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abstract = "Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.",

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T1 - Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

AU - Monkkonen, H

AU - Moilanen, P

AU - Monkkonen, J

AU - Frith, J C

AU - Rogers, M J

AU - Auriola, S

PY - 2000

Y1 - 2000

N2 - Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

AB - Clodronate belongs to the family of bisphosphonates, which are synthetic analogues of pyrophosphate. Bisphosphonates are widely used in the treatment of metabolic bone diseases. Some bisphosphonates, including clodronate, can be metabolized in cells into non-hydrolysable nucleotide analogues. In this paper. we describe a new method for extraction and quantitation of the clodronate metabolite in cell lysates by using ion-pairing HPLC method that is compatible with negative ion electrospray ionization mass spectrometry (ESI-MS). The method was used for detection of the metabolite of clodronate in extracts from RAW 264 macrophage cells after treatment with clodronate. (C) 2000 Elsevier Science B.V. All rights reserved.

KW - clodronate

KW - MOLD DICTYOSTELIUM-DISCOIDEUM

KW - IN-VITRO

KW - BISPHOSPHONATES

KW - AMEBAS

M3 - Article

SN - 1572-6495

VL - 738

SP - 395

EP - 403

JO - Journal of Chromatography B: Biomedical Sciences and Applications

JF - Journal of Chromatography B: Biomedical Sciences and Applications

ER -

Analysis of an adenine nucleotide-containing metabolite of clodronate using ion pair high-performance liquid chromatography-electrospray ionisation mass spectrometry

Abstract

Keywords

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