Analysis of interferon gamma protein expression in zebrafish (Danio rerio)

Sohye Yoon, Ayham Alnabulsi, Steven Bird, Jun Zou, Christopher John Secombes

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18 Citations (Scopus)


IFN-γ is a major effector cytokine, produced to induce type I immune responses. It has been cloned in several fish species including zebrafish, however to date few studies have looked at IFN-γ protein expression and bioactivity in fish. Hence, the current study focused on developing a monoclonal antibody (moAb) against zfIFN-γ. We show that the zfIFN-γ moAb specifically recognises E. coli produced recombinant IFN-γ protein and zfIFN-γ produced in transfected HEK293 cells, by Western blot analysis. Next we analysed the production of the native protein following expression induced by PHA stimulation of leukocytes in vitro or antigen re-stimulation in vivo. We show the IFN-γ protein is produced as a dimer, and that a good correlation exists between transcript expression levels and protein levels.
Original languageEnglish
Pages (from-to)79-86
Number of pages8
JournalFish & Shellfish Immunology
Issue number57
Early online date15 Aug 2016
Publication statusPublished - Oct 2016

Bibliographical note

SY was supported by the Scottish Overseas Research Scholarship Awards Scheme (SORSAS) to undertake a PhD program at the University of Aberdeen. PTL was supported by a PhD studentship from the Ministry of Education, Republic of China (Taiwan). TYW was supported by grants from the Ministry of Science and Technology, Taiwan (NSC 100-2313-B-006-002-MY3 and MOST 103-2313-B-006-004-MY3) to visit Aberdeen. This research was also funded by the European Commission under the 7th Framework Programme for Research and Technological Development (FP7) of the European Union (grant agreement No. 311993 TARGETFISH).


  • Zebrafish
  • IFN-γ
  • Monoclonal antibody
  • Protein expression
  • PHA
  • Antigen re-stimulation


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