Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

K A  Charlton; S  Moyle; A J R  Porter; W J  Harris

Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

K A Charlton, S Moyle, A J R Porter, W J Harris

Medical Sciences

Research output: Contribution to journal › Article

54 Citations (Scopus)

Abstract

We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 10(8) clones from an adult sheep immunized against the hapten atrazine. We have identified eight new V-H gene families (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.

Original language	English
Pages (from-to)	6221-6229
Number of pages	9
Journal	The Journal of Immunology
Volume	164
Publication status	Published - 2000

Keywords

CHAIN VARIABLE REGION
H GENE FAMILY
CHICKEN IMMUNOGLOBULIN GENES
PHAGE-DISPLAY
HEAVY-CHAINS
VH-GENE
GENERATION
CATTLE
DIVERSIFICATION
ORGANIZATION

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title = "Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library",

abstract = "We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 10(8) clones from an adult sheep immunized against the hapten atrazine. We have identified eight new V-H gene families (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.",

keywords = "CHAIN VARIABLE REGION, H GENE FAMILY, CHICKEN IMMUNOGLOBULIN GENES, PHAGE-DISPLAY, HEAVY-CHAINS, VH-GENE, GENERATION, CATTLE, DIVERSIFICATION, ORGANIZATION",

author = "Charlton, {K A} and S Moyle and Porter, {A J R} and Harris, {W J}",

year = "2000",

language = "English",

volume = "164",

pages = "6221--6229",

journal = "The Journal of Immunology",

issn = "0022-1767",

publisher = "American Association of Immunologists",

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TY - JOUR

T1 - Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

AU - Charlton, K A

AU - Moyle, S

AU - Porter, A J R

AU - Harris, W J

PY - 2000

Y1 - 2000

N2 - We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 10(8) clones from an adult sheep immunized against the hapten atrazine. We have identified eight new V-H gene families (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.

AB - We have applied bacteriophage display technology to construct and analyze the diversity of an IgG library of >1 x 10(8) clones from an adult sheep immunized against the hapten atrazine. We have identified eight new V-H gene families (V(H)2-V(H)9) and five new V kappa gene families (V kappa V-V kappa IX). The heavy and kappa light chain variable region gene loci were found to be far more diverse than previously thought.

KW - CHAIN VARIABLE REGION

KW - H GENE FAMILY

KW - CHICKEN IMMUNOGLOBULIN GENES

KW - PHAGE-DISPLAY

KW - HEAVY-CHAINS

KW - VH-GENE

KW - GENERATION

KW - CATTLE

KW - DIVERSIFICATION

KW - ORGANIZATION

M3 - Article

SN - 0022-1767

VL - 164

SP - 6221

EP - 6229

JO - The Journal of Immunology

JF - The Journal of Immunology

ER -

Analysis of the diversity of a sheep antibody repertoire as revealed from a bacteriophage display library

Abstract

Keywords

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