Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis

Georgina Louise Hold; S. E. Pryde; V. J. Russell; E. Furrie; Harry James Flint

doi:10.1111/j.1574-6941.2002.tb00904.x

Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis

Georgina Louise Hold, S. E. Pryde, V. J. Russell, E. Furrie, Harry James Flint

Research output: Contribution to journal › Article › peer-review

310 Citations (Scopus)

Abstract

The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25%, of sequences were closely related (> 97%) to current species type strains, and 28% were less than 97%, related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the First molecular information on the microbial diversity present in human colonic samples. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	33-39
Number of pages	6
Journal	FEMS Microbiology Ecology
Volume	39
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6941.2002.tb00904.x
Publication status	Published - Jan 2002

Keywords

colonic sample
16S rDNA sequencing
microbial diversity
targeted oligonucleotide probes
ribosomal database project
In-situ hybridization
human fecal samples
bacterial-populations
human feces
human gut
RNA
quantification
communities

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title = "Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis",

abstract = "The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25%, of sequences were closely related (> 97%) to current species type strains, and 28% were less than 97%, related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the First molecular information on the microbial diversity present in human colonic samples. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.",

keywords = "colonic sample, 16S rDNA sequencing, microbial diversity, targeted oligonucleotide probes, ribosomal database project, In-situ hybridization, human fecal samples, bacterial-populations, human feces, human gut, RNA, quantification, communities",

author = "Hold, {Georgina Louise} and Pryde, {S. E.} and Russell, {V. J.} and E. Furrie and Flint, {Harry James}",

year = "2002",

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doi = "10.1111/j.1574-6941.2002.tb00904.x",

language = "English",

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AU - Hold, Georgina Louise

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AU - Furrie, E.

AU - Flint, Harry James

PY - 2002/1

Y1 - 2002/1

N2 - The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25%, of sequences were closely related (> 97%) to current species type strains, and 28% were less than 97%, related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the First molecular information on the microbial diversity present in human colonic samples. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

AB - The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25%, of sequences were closely related (> 97%) to current species type strains, and 28% were less than 97%, related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the First molecular information on the microbial diversity present in human colonic samples. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

KW - colonic sample

KW - 16S rDNA sequencing

KW - microbial diversity

KW - targeted oligonucleotide probes

KW - ribosomal database project

KW - In-situ hybridization

KW - human fecal samples

KW - bacterial-populations

KW - human feces

KW - human gut

KW - RNA

KW - quantification

KW - communities

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DO - 10.1111/j.1574-6941.2002.tb00904.x

M3 - Article

SN - 1574-6941

VL - 39

SP - 33

EP - 39

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 1

ER -

Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis

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Keywords

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