Abstract
The acid tolerance of Escherichia coli O157:H7 strains can be overcome by addition of lactate, ethanol, or a combination of the two agents. Killing can be increased by as much as 4 log units in the first 5 min of incubation at pH 3 even for the most acid-tolerant isolates. Exponential-phase, habituated, and stationary-phase cells are all sensitive to incubation with lactate and ethanol. Killing correlates with disruption of the capacity for pH homeostasis. Habituated and, stationary-phase cells can partially offset the effects of the lowering of cytoplasmic pH.
| Original language | English |
|---|---|
| Pages (from-to) | 1308-1311 |
| Number of pages | 4 |
| Journal | Applied and Environmental Microbiology |
| Volume | 65 |
| Publication status | Published - 1999 |
Keywords
- ACID TOLERANCE RESPONSE
- MONOCYTOGENES SCOTT-A
- LISTERIA-MONOCYTOGENES
- SALMONELLA-TYPHIMURIUM
- INTRACELLULAR PH
- APPLE CIDER
- SURVIVAL
- GROWTH
- HABITUATION
- RESISTANCE
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