Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

A M  O'Dowd; A E  Ellis; C J  Secombes

Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

A M O'Dowd, A E Ellis, C J Secombes

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article

7 Citations (Scopus)

Abstract

Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg(+) and sIg(-) enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg(+) and sIg(-) enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes. (C) 1999 Elsevier Science B.V. All rights reserved.

Original language	English
Pages (from-to)	149-157
Number of pages	9
Journal	Veterinary Immunology and Immunopathology
Volume	68
Publication status	Published - 1999

Keywords

Atlantic salmon
B lymphocytes
flow cytometry
immune complexes
macrophages
neutrophils
FC-GAMMA RECEPTORS
RAINBOW-TROUT
ONCORHYNCHUS-MYKISS
PROLIFERATIVE RESPONSES
AEROMONAS-SALMONICIDA
MONOCLONAL-ANTIBODIES
LEUKOCYTES
IGM
PHAGOCYTOSIS
EXPRESSION

Cite this

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title = "Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes",

abstract = "Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg(+) and sIg(-) enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg(+) and sIg(-) enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes. (C) 1999 Elsevier Science B.V. All rights reserved.",

keywords = "Atlantic salmon, B lymphocytes, flow cytometry, immune complexes, macrophages, neutrophils, FC-GAMMA RECEPTORS, RAINBOW-TROUT, ONCORHYNCHUS-MYKISS, PROLIFERATIVE RESPONSES, AEROMONAS-SALMONICIDA, MONOCLONAL-ANTIBODIES, LEUKOCYTES, IGM, PHAGOCYTOSIS, EXPRESSION",

author = "O'Dowd, {A M} and Ellis, {A E} and Secombes, {C J}",

year = "1999",

language = "English",

volume = "68",

pages = "149--157",

journal = "Veterinary Immunology and Immunopathology",

issn = "0165-2427",

publisher = "Elsevier",

}

TY - JOUR

T1 - Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

AU - O'Dowd, A M

AU - Ellis, A E

AU - Secombes, C J

PY - 1999

Y1 - 1999

N2 - Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg(+) and sIg(-) enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg(+) and sIg(-) enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes. (C) 1999 Elsevier Science B.V. All rights reserved.

AB - Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg(+) and sIg(-) enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg(+) and sIg(-) enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes. (C) 1999 Elsevier Science B.V. All rights reserved.

KW - Atlantic salmon

KW - B lymphocytes

KW - flow cytometry

KW - immune complexes

KW - macrophages

KW - neutrophils

KW - FC-GAMMA RECEPTORS

KW - RAINBOW-TROUT

KW - ONCORHYNCHUS-MYKISS

KW - PROLIFERATIVE RESPONSES

KW - AEROMONAS-SALMONICIDA

KW - MONOCLONAL-ANTIBODIES

KW - LEUKOCYTES

KW - IGM

KW - PHAGOCYTOSIS

KW - EXPRESSION

M3 - Article

SN - 0165-2427

VL - 68

SP - 149

EP - 157

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

ER -

Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

Abstract

Keywords

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