Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion

Bryan Moreno-Chamba; Julio Salazar-Bermeo; María Concepción Martínez-Madrid; Victoria Lizama; Francisco Martín-Bermudo; Genoveva Berná; Madalina Neacsu; Domingo Saura; Nuria Martí; Manuel Valero

doi:10.1016/j.lwt.2021.113011

Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion

Bryan Moreno-Chamba, Julio Salazar-Bermeo, María Concepción Martínez-Madrid, Victoria Lizama, Francisco Martín-Bermudo, Genoveva Berná, Madalina Neacsu, Domingo Saura, Nuria Martí, Manuel Valero^*

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

Research output: Contribution to journal › Article › peer-review

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Abstract

Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.

Original language	English
Article number	113011
Number of pages	11
Journal	LWT
Volume	156
Early online date	24 Dec 2021
DOIs	https://doi.org/10.1016/j.lwt.2021.113011
Publication status	Published - 15 Feb 2022

Bibliographical note

Funding Information:
This research was financed by Ministry of Science Innovation and Universities through the funded project ‘Industrial symbiosis in the integral use of persimmon (Diospyros kaki); Bioeconomy example '(CTM2017-88978-R). The authors gratefully acknowledge Miss Laura Agulló and Mitra Sol Technologies S.L. for the given technical assistance.

Keywords

Antibacterial synergy testing
Antimicrobial activity
Dietary polysaccharides
Persimmon byproducts
Prebiotic activity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Morena_Chamba_et_al_Bound_galloylated_LWT_vor
© 2021 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license https://creativecommons.org/licenses/by-nc-nd/4.0/
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Cite this

Moreno-Chamba, B., Salazar-Bermeo, J., Martínez-Madrid, M. C., Lizama, V., Martín-Bermudo, F., Berná, G., Neacsu, M., Saura, D., Martí, N., & Valero, M. (2022). Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion. LWT, 156, [113011]. https://doi.org/10.1016/j.lwt.2021.113011

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title = "Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion",

abstract = "Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.",

keywords = "Antibacterial synergy testing, Antimicrobial activity, Dietary polysaccharides, Persimmon byproducts, Prebiotic activity",

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note = "Funding Information: This research was financed by Ministry of Science Innovation and Universities through the funded project {\textquoteleft}Industrial symbiosis in the integral use of persimmon (Diospyros kaki); Bioeconomy example '(CTM2017-88978-R). The authors gratefully acknowledge Miss Laura Agull{\'o} and Mitra Sol Technologies S.L. for the given technical assistance. ",

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T1 - Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion

AU - Moreno-Chamba, Bryan

AU - Salazar-Bermeo, Julio

AU - Martínez-Madrid, María Concepción

AU - Lizama, Victoria

AU - Martín-Bermudo, Francisco

AU - Berná, Genoveva

AU - Neacsu, Madalina

AU - Saura, Domingo

AU - Martí, Nuria

AU - Valero, Manuel

N1 - Funding Information: This research was financed by Ministry of Science Innovation and Universities through the funded project ‘Industrial symbiosis in the integral use of persimmon (Diospyros kaki); Bioeconomy example '(CTM2017-88978-R). The authors gratefully acknowledge Miss Laura Agulló and Mitra Sol Technologies S.L. for the given technical assistance.

PY - 2022/2/15

Y1 - 2022/2/15

N2 - Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.

AB - Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.

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KW - Antimicrobial activity

KW - Dietary polysaccharides

KW - Persimmon byproducts

KW - Prebiotic activity

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DO - 10.1016/j.lwt.2021.113011

M3 - Article

AN - SCOPUS:85121848928

SN - 0023-6438

VL - 156

JO - LWT

JF - LWT

M1 - 113011

ER -

Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion

Abstract

Bibliographical note

Keywords

UN SDGs

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