Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion

Bryan Moreno-Chamba, Julio Salazar-Bermeo, María Concepción Martínez-Madrid, Victoria Lizama, Francisco Martín-Bermudo, Genoveva Berná, Madalina Neacsu, Domingo Saura, Nuria Martí, Manuel Valero*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
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Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.

Original languageEnglish
Article number113011
Number of pages11
Early online date24 Dec 2021
Publication statusPublished - 15 Feb 2022

Bibliographical note

Funding Information:
This research was financed by Ministry of Science Innovation and Universities through the funded project ‘Industrial symbiosis in the integral use of persimmon (Diospyros kaki); Bioeconomy example '(CTM2017-88978-R). The authors gratefully acknowledge Miss Laura Agulló and Mitra Sol Technologies S.L. for the given technical assistance.


  • Antibacterial synergy testing
  • Antimicrobial activity
  • Dietary polysaccharides
  • Persimmon byproducts
  • Prebiotic activity


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