Breakdown of diazotized proteins and synthetic substrates by rumen bacterial proteases

R. J. Wallace, J. Kopecny

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21 Citations (Scopus)


Several different kinds of substrate were used to investigate the proteolytic activity of rumen bacteria and of proteases released from rumen bacteria by blending ("coat proteases"). These substrates included diazotized feed proteins and diazotized soluble and insoluble pure proteins. It was concluded that, while solubility was an important factor, the secondary and tertiary structure of a protein had a major influence on its rate of digestion. The resistance of elastin congo red to digestion indicated that similar fibrous proteins in plant material might resist proteolytic attack by rumen bacteria. Coat proteases had a broad specificity, including several exo- and endopeptidase activities, as determined by using synthetic peptide substrates.
Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalApplied and Environmental Microbiology
Issue number1
Publication statusPublished - Jan 1983

Bibliographical note

Copyright 2004 Elsevier B.V., All rights reserved.


  • proteinase


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