Bypassing the proline/thiazoline requirement of the macrocyclase PatG

E Oueis, H Stevenson, M Jaspars, N J Westwood, J H Naismith

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18 Citations (Scopus)
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Biocatalysis is a fast developing field in which an enzyme's natural capabilities are harnessed or engineered for synthetic chemistry. The enzyme PatG is an extremely promiscuous macrocyclase enzyme tolerating both non-natural amino acids and non-amino acids within the substrate. It does, however, require a proline or thiazoline at the C-terminal position of the core peptide which means the final product must contain this group. Here, we show guided by structural insight we have identified two synthetic routes, triazole and a double cysteine, that circumvent this requirement. With the triazole, we show PatGmac can macrocyclise substrates that do not contain any amino acids in the final product.

Original languageEnglish
Pages (from-to)12274-12277
Number of pages4
JournalChemical Communications
Issue number91
Early online date1 Nov 2017
Publication statusPublished - 25 Nov 2017


  • Journal Article


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