Cardiomyocyte Differentiation from Human Embryonic Stem Cells

Silvia Mazzotta; Adam T Lynch; Stefan Hoppler

doi:10.1007/978-1-4939-8597-5_5

Cardiomyocyte Differentiation from Human Embryonic Stem Cells

Silvia Mazzotta
, Adam T Lynch
, Stefan Hoppler

Research output: Chapter in Book/Report/Conference proceeding › Chapter

6 Citations (Scopus)

Abstract

In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modelling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced Pluripotent Stem Cells into cardiomyocytes.

Original language	English
Title of host publication	Experimental Models of Cardiovascular Diseases
Editors	K Ishikawa
Publisher	Springer
Pages	67-78
Number of pages	12
ISBN (Electronic)	978-1-4939-8597-5
ISBN (Print)	978-1-4939-8596-8
DOIs	https://doi.org/10.1007/978-1-4939-8597-5_5
Publication status	Published - 2018

Publication series

Name	Methods in Molecular Biology
Publisher	Springer
ISSN (Electronic)	1064-3745

Bibliographical note

We are grateful to Po-Lin So and Bruce Conklin (Gladstone Institutes) for providing a modified version of the Lian et al., protocol cardiomyocyte differentiation from human ES cells. We thank Kate Watt, and Yvonne Turnbull (University of Aberdeen), for lab management and technical support, Rory J Bonner (University of Aberdeen) for providing images, the British Heart Foundation (PG/12/75/29851) for research funding, and the Institute of Medical Sciences (University of Aberdeen) for PhD studentship funding.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

human embryonic stem cells
human pluripotent stem cells
human induced pluripotent stem cells
human cardiomyocytes
human heart development
in vitro differentiation
Wnt signalling

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Cite this

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title = "Cardiomyocyte Differentiation from Human Embryonic Stem Cells",

abstract = "In vitro generated human cardiomyocytes hold the ultimate promise for heart patients for repair of injured or diseased myocardium, but they also provide experimental models for studying normal cardiomyocyte development, for disease modelling and for drug development. Here we provide reliable protocols for differentiation of human embryonic stem cells into functional cardiomyocytes, together with Notes about trouble shooting and optimising such protocols for specific cell lines. This chapter also briefly discusses other published protocols and those further adapted for differentiation of induced Pluripotent Stem Cells into cardiomyocytes.",

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AU - Lynch, Adam T

AU - Hoppler, Stefan

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KW - human induced pluripotent stem cells

KW - human cardiomyocytes

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KW - in vitro differentiation

KW - Wnt signalling

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DO - 10.1007/978-1-4939-8597-5_5

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ER -

Cardiomyocyte Differentiation from Human Embryonic Stem Cells

Abstract

Publication series

Bibliographical note

UN SDGs

Keywords

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