Abstract
The diversity of endobacteria associated with ectomycorrhizas of Suillus variegatus and Tomentellopsis submollis, in two Corsican pine (Pinus nigra) stands was analysed by cultivation-dependent and cultivation-independent molecular methods. Denaturing gradient gel electrophoresis (DGGE) analysis revealed the cultivable endobacterial communities associated with S. variegatus were similar within the same stand. The most abundant cultivable bacterial species belonged to the genera Pseudomonas and Burkholderia. Cultivation-independent molecular analysis indicated that the structure of the endobacterial communities in ectomycorrhizas was consistent across all samples regardless of ECM fungal species or the pine stand from which the samples were collected. However, comparison between rDNA- and rRNA-derived DGGE gels showed that metabolically active endobacterial species were not always detected in rDNA-based profiles. Clone libraries constructed from rRNA molecules indicated that Pseudomonas and Burkholderia spp. were metabolically active bacteria. As some of the most abundant cultivable bacteria, including Bacillus/Paenibacillus spp., were not detected in cultivation-independent DGGE profiles, a combination of cultivation-dependent and -independent approaches provided a more complete assessment of the diversity of endobacteria associated with ectomycorrhizas. (c) 2006 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 891-899 |
| Number of pages | 9 |
| Journal | Soil Biology and Biochemistry |
| Volume | 39 |
| Issue number | 4 |
| Early online date | 6 Dec 2006 |
| DOIs | |
| Publication status | Published - Apr 2007 |
Keywords
- endobacteria
- 16S rRNA
- metabolically active bacteria
- ectomycorrhiza
- gradient gel-electrophoresis
- 16S ribosomal-RNA
- tuber-borchii vittad
- in-situ detection
- pinus-sylvestris
- bacterial community
- helper bacteria
- populations
- endophyte
- growth