Characterisation of ribosomal satellite in total nuclear DNA from Physarum polycephalum

Norman Hardman; Peter L. Jack; Alistair J P Brown; Alan McLachlan

doi:10.1016/0005-2787(79)90101-1

Characterisation of ribosomal satellite in total nuclear DNA from Physarum polycephalum

Norman Hardman^*
, Peter L. Jack
, Alistair J P Brown
, Alan McLachlan

^*Corresponding author for this work

Medical Sciences

University of Aberdeen

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

The distinctive properties of satellite DNA molecules containing the genes for ribosomal RNA in Physarum polycephalum permits their identification in total, unfractionated nuclear DNA in the foldback form, after denaturation and fast annealing. Using the electron microscope the location and properties of three characteristic regions containing tandemly-repeated, inverted sequences have been investigated. At least two additional regions, also containing tandem repeats, are shown to be present and located towards each end of the rDNA molecule, at a site adjacent to the segment coding for the 26 S rRNA. All the regions which contain tandem repeats are composed of sequences which, within experimental error, appear to share a common unit repeat length of about 90 nucleotides.

Original language	English
Pages (from-to)	365-376
Number of pages	12
Journal	BBA Section Nucleic Acids And Protein Synthesis
Volume	562
Issue number	3
DOIs	https://doi.org/10.1016/0005-2787(79)90101-1
Publication status	Published - 24 May 1979

Funding

The authors are grateful to Henry Eichelberger and Doris Duncan for preparation of micrographs. P.L.J. and A.J.P.B. thank the Science Research Council for the award of studentships. This work is supported by the Medical Research Council and The Scottish Hospitals Endowment Research Trust.

Keywords

(Physarum)
rDNA
rRNA
Satellite DNA

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title = "Characterisation of ribosomal satellite in total nuclear DNA from Physarum polycephalum",

abstract = "The distinctive properties of satellite DNA molecules containing the genes for ribosomal RNA in Physarum polycephalum permits their identification in total, unfractionated nuclear DNA in the foldback form, after denaturation and fast annealing. Using the electron microscope the location and properties of three characteristic regions containing tandemly-repeated, inverted sequences have been investigated. At least two additional regions, also containing tandem repeats, are shown to be present and located towards each end of the rDNA molecule, at a site adjacent to the segment coding for the 26 S rRNA. All the regions which contain tandem repeats are composed of sequences which, within experimental error, appear to share a common unit repeat length of about 90 nucleotides.",

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AU - Hardman, Norman

AU - Jack, Peter L.

AU - Brown, Alistair J P

AU - McLachlan, Alan

PY - 1979/5/24

Y1 - 1979/5/24

N2 - The distinctive properties of satellite DNA molecules containing the genes for ribosomal RNA in Physarum polycephalum permits their identification in total, unfractionated nuclear DNA in the foldback form, after denaturation and fast annealing. Using the electron microscope the location and properties of three characteristic regions containing tandemly-repeated, inverted sequences have been investigated. At least two additional regions, also containing tandem repeats, are shown to be present and located towards each end of the rDNA molecule, at a site adjacent to the segment coding for the 26 S rRNA. All the regions which contain tandem repeats are composed of sequences which, within experimental error, appear to share a common unit repeat length of about 90 nucleotides.

AB - The distinctive properties of satellite DNA molecules containing the genes for ribosomal RNA in Physarum polycephalum permits their identification in total, unfractionated nuclear DNA in the foldback form, after denaturation and fast annealing. Using the electron microscope the location and properties of three characteristic regions containing tandemly-repeated, inverted sequences have been investigated. At least two additional regions, also containing tandem repeats, are shown to be present and located towards each end of the rDNA molecule, at a site adjacent to the segment coding for the 26 S rRNA. All the regions which contain tandem repeats are composed of sequences which, within experimental error, appear to share a common unit repeat length of about 90 nucleotides.

KW - (Physarum)

KW - rDNA

KW - rRNA

KW - Satellite DNA

UR - https://www.scopus.com/pages/publications/0018799812

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DO - 10.1016/0005-2787(79)90101-1

M3 - Article

C2 - 454607

AN - SCOPUS:0018799812

SN - 0005-2787

VL - 562

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EP - 376

JO - BBA Section Nucleic Acids And Protein Synthesis

JF - BBA Section Nucleic Acids And Protein Synthesis

IS - 3

ER -

Characterisation of ribosomal satellite in total nuclear DNA from Physarum polycephalum

Abstract

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Keywords

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