Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula)

Ronggai Li, Anthony K Redmond, Tiehui Wang, Steve Bird, Helen Dooley, Chris J Secombes

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)
42 Downloads (Pure)


The tumour necrosis factor superfamily (TNFSF) members CD40L and BAFF play critical roles in mammalian B cell survival, proliferation and maturation, however little is known about these key cytokines in the oldest jawed vertebrates, the cartilaginous fishes. Here we report the cloning of CD40L and BAFF orthologues (designated ScCD40L and ScBAFF) in the small-spotted catshark (Scyliorhinus canicula). As predicted both proteins are type II membrane-bound proteins with a TNF homology domain in their extracellular region and both are highly expressed in shark immune tissues. ScCD40L transcript levels correlate with those of TCRα and transcription of both genes is modulated in peripheral blood leukocytes following in vitro stimulation. Although a putative CD40L orthologue was identified in the elephant shark genome the work herein is the first molecular characterisation and transcriptional analysis of CD40L in a cartilaginous fish. ScBAFF was also cloned and its transcription characterised in an attempt to resolve the discrepancies observed between spiny dogfish BAFF and bamboo shark BAFF in previously published studies.

Original languageEnglish
Pages (from-to)381-389
Number of pages9
JournalFish & Shellfish Immunology
Issue number1
Early online date16 Sept 2015
Publication statusPublished - 1 Nov 2015

Bibliographical note


This work was supported financially by a BBSRC Industrial CASE Studentship (BB/G01731X/1) with Pfizer to RL.


  • CD40L
  • BAFF
  • shark
  • cartilaginous fish


Dive into the research topics of 'Characterisation of the TNF superfamily members CD40L and BAFF in the small-spotted catshark (Scyliorhinus canicula)'. Together they form a unique fingerprint.

Cite this