Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish

Jun Wang; Tiehui Wang; Ottavia Benedicenti; Catherine Collins; Kaiyu Wang; Christopher J. Secombes; Jun Zou

doi:10.1016/j.dci.2016.11.007

Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish

Jun Wang
, Tiehui Wang
, Ottavia Benedicenti
, Catherine Collins
, Kaiyu Wang
, Christopher J. Secombes
, Jun Zou

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.

Original language	English
Pages (from-to)	2-14
Number of pages	13
Journal	Developmental and Comparative Immunology
Volume	80
Early online date	23 Nov 2016
DOIs	https://doi.org/10.1016/j.dci.2016.11.007
Publication status	Published - Mar 2018

Bibliographical note

Acknowledgements
This work is supported by the Royal Society of Edinburgh. JW's visit to the Scottish Fish Immunology Research Centre was funded by the China Scholarship Council (CSC). Generation of P. perurans samples was supported through a PhD studentship from the Marine Collaboration Research Forum (MarCRF), which is a collaboration between the University of Aberdeen and Marine Scotland Science, and through Scottish Government grant AQ0080.

Keywords

ZBTB46
DC-SCRIPT/ZNF366
Dendritic cell
Transcription factor
Gene expression
Interferon

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title = "Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish",

abstract = "ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.",

keywords = "ZBTB46, DC-SCRIPT/ZNF366, Dendritic cell, Transcription factor, Gene expression, Interferon",

author = "Jun Wang and Tiehui Wang and Ottavia Benedicenti and Catherine Collins and Kaiyu Wang and Secombes, \{Christopher J.\} and Jun Zou",

note = "Acknowledgements This work is supported by the Royal Society of Edinburgh. JW's visit to the Scottish Fish Immunology Research Centre was funded by the China Scholarship Council (CSC). Generation of P. perurans samples was supported through a PhD studentship from the Marine Collaboration Research Forum (MarCRF), which is a collaboration between the University of Aberdeen and Marine Scotland Science, and through Scottish Government grant AQ0080.",

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doi = "10.1016/j.dci.2016.11.007",

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AU - Benedicenti, Ottavia

AU - Collins, Catherine

AU - Wang, Kaiyu

AU - Secombes, Christopher J.

AU - Zou, Jun

N1 - Acknowledgements This work is supported by the Royal Society of Edinburgh. JW's visit to the Scottish Fish Immunology Research Centre was funded by the China Scholarship Council (CSC). Generation of P. perurans samples was supported through a PhD studentship from the Marine Collaboration Research Forum (MarCRF), which is a collaboration between the University of Aberdeen and Marine Scotland Science, and through Scottish Government grant AQ0080.

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AB - ZBTB46 and DC-SCRIPT/ZNF366 are two zinc finger transcription factors that play important roles in regulating differentiation of dendritic cells in mammals. In this study, the ZBTB46 and DC-SCRIPT/ZNF366 homologues were identified in rainbow trout Oncorhynchus mykiss and their expression analysed in vivo and in vitro. As transcription factors, they are well conserved in sequence, genomic organisation and gene synteny. Their expression was differentially modulated by bacterial and viral PAMPs in the monocyte/macrophage like cell line RTS-11, in primary head kidney (HK) macrophages, and in HK macrophages cultured with IL-4/13A. In the RTS-11 cells and primary HK macrophages, all the ZBTB46 and DC-SCRIPT/ZNF366 homologues were down-regulated by interferon gamma (type II IFN) but unaffected by IFN2 (type I IFN), administered as recombinant proteins to cell cultures. In fish gills, infection with amoebae (Paramoebae perurans) resulted in reduction of ZBTB46 and DC-SCRIPT/ZNF366 expression in Atlantic salmon Salmo salar, whilst infection with Yersinia ruckeri induced gene expression in rainbow trout.

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KW - DC-SCRIPT/ZNF366

KW - Dendritic cell

KW - Transcription factor

KW - Gene expression

KW - Interferon

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ER -

Characterisation of ZBTB46 and DC-SCRIPT/ZNF366 in rainbow trout, transcription factors potentially involved in dendritic cell maturation and activation in fish

Abstract

Bibliographical note

Keywords

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