Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

CG Mowat; D Leys; KJ McLean; SL Rivers; A Richmond; AW Munro; MO Lombardia; PM Alzari; GA Reid; Stephen K. Chapman; MD Walkinshaw

doi:10.1107/S0907444902002676

Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

CG Mowat, D Leys, KJ McLean, SL Rivers, A Richmond, AW Munro, MO Lombardia, PM Alzari, GA Reid, Stephen K. Chapman, MD Walkinshaw^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 Angstrom. Native data have been collected to 1.6 Angstrom resolution and Hg-derivative data to 2.5 Angstrom resolution using a synchrotron-radiation source.

Original language	English
Pages (from-to)	704-705
Number of pages	2
Journal	Acta crystallographica. Section D, Biological crystallography
Volume	58
DOIs	https://doi.org/10.1107/S0907444902002676
Publication status	Published - Apr 2002
Externally published	Yes

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Mowat, CG., Leys, D., McLean, KJ., Rivers, SL., Richmond, A., Munro, AW., Lombardia, MO., Alzari, PM., Reid, GA., Chapman, S. K., & Walkinshaw, MD. (2002). Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis. Acta crystallographica. Section D, Biological crystallography, 58, 704-705. https://doi.org/10.1107/S0907444902002676

Mowat, CG, Leys, D, McLean, KJ, Rivers, SL, Richmond, A, Munro, AW, Lombardia, MO, Alzari, PM, Reid, GA, Chapman, SK & Walkinshaw, MD 2002, 'Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis', Acta crystallographica. Section D, Biological crystallography, vol. 58, pp. 704-705. https://doi.org/10.1107/S0907444902002676

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title = "Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis",

abstract = "The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 Angstrom. Native data have been collected to 1.6 Angstrom resolution and Hg-derivative data to 2.5 Angstrom resolution using a synchrotron-radiation source.",

author = "CG Mowat and D Leys and KJ McLean and SL Rivers and A Richmond and AW Munro and MO Lombardia and PM Alzari and GA Reid and Chapman, {Stephen K.} and MD Walkinshaw",

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AU - Mowat, CG

AU - Leys, D

AU - McLean, KJ

AU - Rivers, SL

AU - Richmond, A

AU - Munro, AW

AU - Lombardia, MO

AU - Alzari, PM

AU - Reid, GA

AU - Chapman, Stephen K.

AU - Walkinshaw, MD

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AB - The product of the Rv2276 gene of Mycobacterium tuberculosis is a cytochrome P450 (P450 MT2, CYP121) which has been shown to bind tightly to a range of azole-based antifungal drugs (e.g. miconazole, clotrimazole). These drugs are potent inhibitors of mycobacterial growth, suggesting that P450 MT2 (CYP121) may be a potential drug target. The enzyme has been overexpressed in Escherichia coli and crystallized by the hanging-drop method. Crystals of P450 MT2 (CYP121) belong to the hexagonal space group P6(1)22 or P6(5)22, with unit-cell parameters a = b = 78.3, c = 265.6 Angstrom. Native data have been collected to 1.6 Angstrom resolution and Hg-derivative data to 2.5 Angstrom resolution using a synchrotron-radiation source.

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DO - 10.1107/S0907444902002676

M3 - Article

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VL - 58

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JO - Acta crystallographica. Section D, Biological crystallography

JF - Acta crystallographica. Section D, Biological crystallography

ER -

Crystallization and preliminary crystallographic analysis of a novel cytochrome P450 from Mycobacterium tuberculosis

Abstract

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