Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

M. Radwanska; S. Magez; H. Perry-O'Keefe; H. Stender; J. Coull; Jeremy M Sternberg; P. Buscher; J. J. Hyldig-Nielsen

doi:10.1128/JCM.40.11.4295-4297.2002

Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

M. Radwanska, S. Magez, H. Perry-O'Keefe, H. Stender, J. Coull, Jeremy M Sternberg, P. Buscher, J. J. Hyldig-Nielsen

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

34 Citations (Scopus)

Abstract

We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

Original language	English
Pages (from-to)	4295-4297
Number of pages	2
Journal	Journal of Clinical Microbiology
Volume	40
Issue number	11
DOIs	https://doi.org/10.1128/JCM.40.11.4295-4297.2002
Publication status	Published - 2002

Keywords

SLEEPING-SICKNESS SUSPECTS
POLYMERASE-CHAIN-REACTION
BRUCEI-GAMBIENSE
PCR AMPLIFICATION
EXPRESSION
DIAGNOSIS
CAMEROON
UGANDA
CATT

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1128/JCM.40.11.4295-4297.2002

Cite this

@article{178d9efc629e43e3a2e53b52cb1aa577,

title = "Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes",

abstract = "We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.",

keywords = "SLEEPING-SICKNESS SUSPECTS, POLYMERASE-CHAIN-REACTION, BRUCEI-GAMBIENSE, PCR AMPLIFICATION, EXPRESSION, DIAGNOSIS, CAMEROON, UGANDA, CATT",

author = "M. Radwanska and S. Magez and H. Perry-O'Keefe and H. Stender and J. Coull and Sternberg, {Jeremy M} and P. Buscher and Hyldig-Nielsen, {J. J.}",

year = "2002",

doi = "10.1128/JCM.40.11.4295-4297.2002",

language = "English",

volume = "40",

pages = "4295--4297",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "11",

}

TY - JOUR

T1 - Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

AU - Radwanska, M.

AU - Magez, S.

AU - Perry-O'Keefe, H.

AU - Stender, H.

AU - Coull, J.

AU - Sternberg, Jeremy M

AU - Buscher, P.

AU - Hyldig-Nielsen, J. J.

PY - 2002

Y1 - 2002

N2 - We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

AB - We have developed a rapid and easy to perform fluorescence in situ hybridization test that allows specific identification of trypanosomes from the subgenus Trypanozoon, using peptide nucleic acid probes. Probes were designed to target subgenus-specific sequences on the multiple-copy 18S rRNA, greatly facilitating the detection of a single trypanosome.

KW - SLEEPING-SICKNESS SUSPECTS

KW - POLYMERASE-CHAIN-REACTION

KW - BRUCEI-GAMBIENSE

KW - PCR AMPLIFICATION

KW - EXPRESSION

KW - DIAGNOSIS

KW - CAMEROON

KW - UGANDA

KW - CATT

U2 - 10.1128/JCM.40.11.4295-4297.2002

DO - 10.1128/JCM.40.11.4295-4297.2002

M3 - Article

SN - 0095-1137

VL - 40

SP - 4295

EP - 4297

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 11

ER -

Direct detection and identification of African trypanosomes by fluorescence in situ hybridization with peptide nucleic acid probes

Abstract

Keywords

UN SDGs

Access to Document

Fingerprint

Cite this