Dot-blotting--a novel screening assay for antibodies in hybridoma cultures

Jeremy Sternberg, Peter Jeppesen

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)


A novel solid phase radioimmunoassay using nitrocellulose filter, is described. The method is of particular value in screening hybridoma cultures for monoclonal antibodies against chromosomal protein. Also it permits detection of antibodies directed against epitopes stable to SDS denaturation
Original languageEnglish
Pages (from-to)39-43
Number of pages5
JournalJournal of Immunological Methods
Issue number1-2
Publication statusPublished - Nov 1983


  • radioimmunoassay
  • monoclonal antibody
  • chromosome protein


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