Expression analysis of limb element markers during mouse embryonic development

Alexandra Rafipay, Amanda L. R. Berg, Lynda Erskine, Neil Vargesson* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)
9 Downloads (Pure)


Background: While data regarding expression of limb element and tissue markers during normal mouse limb development exists, few studies show expression patterns in upper and lower limbs throughout key limb development stages. A comparison to normal developmental events is essential when analysing development of the limb in mutant mice models.
Results: Expression patterns of the joint marker Gdf5, tendon and ligament marker Scleraxis, early muscle marker MyoD1, and blood vessel marker Cadherin5 (Cdh5) are presented during the most active phases of embryonic mouse limb patterning. Anti-neurofilament staining of developing nerves in the fore- and hind-limbs and cartilage formation and progression also is described.
Conclusions: This study demonstrates and describes a range of key morphological markers, and methods, which together, can be used to assess normal and abnormal limb development.
Original languageEnglish
Pages (from-to)1217-1226
Number of pages10
JournalDevelopmental Dynamics
Issue number11
Early online date7 Nov 2018
Publication statusPublished - 20 Nov 2018

Bibliographical note

The authors declare no conflicts of interest. The authors thank Yolanda Ramirez Casas and J. Martin Collinson for technical advice and past and present lab staff for helpful discussions.


  • gene expression
  • limb development
  • Forelimb
  • hindlimb
  • cartilage
  • nerves
  • alcian blue/alizarin red


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