Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

G  Khodakaramian; S  Lissenden; B  Gust; L  Moir; P A  Hoskisson; K F  Chater; M C M  Smith

doi:10.1093/nar/gnj019

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

G Khodakaramian, S Lissenden, B Gust, L Moir, P A Hoskisson, K F Chater, M C M Smith

Medical Sciences

Research output: Contribution to journal › Article › peer-review

30 Citations (Scopus)

Abstract

We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.

Original language	English
Number of pages	5
Journal	Nucleic Acids Research
Volume	34
DOIs	https://doi.org/10.1093/nar/gnj019
Publication status	Published - 2006

Keywords

COELICOLOR A3(2)
LYTIC DEVELOPMENT
ESCHERICHIA-COLI
EARLY REGION
PHI-C31
SEQUENCE
CLONING
GENOME
GENES
SITE

Access to Document

10.1093/nar/gnj019

Cite this

@article{873dc7082655489b82c8c9c9b8145d6a,

title = "Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces",

abstract = "We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.",

keywords = "COELICOLOR A3(2), LYTIC DEVELOPMENT, ESCHERICHIA-COLI, EARLY REGION, PHI-C31, SEQUENCE, CLONING, GENOME, GENES, SITE",

author = "G Khodakaramian and S Lissenden and B Gust and L Moir and Hoskisson, {P A} and Chater, {K F} and Smith, {M C M}",

year = "2006",

doi = "10.1093/nar/gnj019",

language = "English",

volume = "34",

journal = "Nucleic Acids Research",

issn = "0305-1048",

publisher = "Oxford University Press",

}

TY - JOUR

T1 - Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

AU - Khodakaramian, G

AU - Lissenden, S

AU - Gust, B

AU - Moir, L

AU - Hoskisson, P A

AU - Chater, K F

AU - Smith, M C M

PY - 2006

Y1 - 2006

N2 - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.

AB - We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage phi C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or PgI in S. coelicolor. Cre-phage was also used for marker deletion in other strains of S. coelicolor.

KW - COELICOLOR A3(2)

KW - LYTIC DEVELOPMENT

KW - ESCHERICHIA-COLI

KW - EARLY REGION

KW - PHI-C31

KW - SEQUENCE

KW - CLONING

KW - GENOME

KW - GENES

KW - SITE

U2 - 10.1093/nar/gnj019

DO - 10.1093/nar/gnj019

M3 - Article

SN - 0305-1048

VL - 34

JO - Nucleic Acids Research

JF - Nucleic Acids Research

ER -

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces

Abstract

Keywords

Access to Document

Fingerprint

Cite this