Generation of human osteoclasts from peripheral blood

Kim Henriksen; Morten A Karsdal; Adam Taylor; Denise Tosh; Fraser Coxon

doi:10.1007/978-1-61779-415-5_11

Generation of human osteoclasts from peripheral blood

Kim Henriksen, Morten A Karsdal, Adam Taylor, Denise Tosh, Fraser Coxon

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

Original language	English
Pages (from-to)	159-175
Number of pages	17
Journal	Methods in Molecular Biology
Volume	816
Early online date	14 Oct 2011
DOIs	https://doi.org/10.1007/978-1-61779-415-5_11
Publication status	Published - 2012

Keywords

acid phosphatase
antigens, CD14
blood buffy coat
bone resorption
cell culture techniques
cell differentiation
collagen
cryopreservation
humans
integrin alphaVbeta3
isoenzymes
leukocytes, mononuclear
macrophage colony-stimulating factor
macrophages
osteoclasts
osteogenesis
RANK ligand

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10.1007/978-1-61779-415-5_11

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title = "Generation of human osteoclasts from peripheral blood",

abstract = "Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.",

keywords = "acid phosphatase, antigens, CD14, blood buffy coat, bone resorption, cell culture techniques, cell differentiation, collagen, cryopreservation, humans, integrin alphaVbeta3, isoenzymes, leukocytes, mononuclear, macrophage colony-stimulating factor, macrophages, osteoclasts, osteogenesis, RANK ligand",

author = "Kim Henriksen and Karsdal, {Morten A} and Adam Taylor and Denise Tosh and Fraser Coxon",

year = "2012",

doi = "10.1007/978-1-61779-415-5_11",

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journal = "Methods in Molecular Biology",

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T1 - Generation of human osteoclasts from peripheral blood

AU - Henriksen, Kim

AU - Karsdal, Morten A

AU - Taylor, Adam

AU - Tosh, Denise

AU - Coxon, Fraser

PY - 2012

Y1 - 2012

N2 - Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

AB - Osteoclasts are multi-nucleated cells that have the unique ability to resorb calcified bone matrix. They derive from haematopoietic precursor cells, and can be generated in vitro by stimulation of peripheral blood mononuclear cells with the cytokines M-CSF and RANKL. In this chapter, we describe the method for generating human osteoclast from peripheral blood or buffy coats, as well as methods for studying both the differentiation and resorbing activity of these cells.

KW - acid phosphatase

KW - antigens, CD14

KW - blood buffy coat

KW - bone resorption

KW - cell culture techniques

KW - cell differentiation

KW - collagen

KW - cryopreservation

KW - humans

KW - integrin alphaVbeta3

KW - isoenzymes

KW - leukocytes, mononuclear

KW - macrophage colony-stimulating factor

KW - macrophages

KW - osteoclasts

KW - osteogenesis

KW - RANK ligand

U2 - 10.1007/978-1-61779-415-5_11

DO - 10.1007/978-1-61779-415-5_11

M3 - Article

C2 - 22130928

SN - 1064-3745

VL - 816

SP - 159

EP - 175

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

ER -

Generation of human osteoclasts from peripheral blood

Abstract

Keywords

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