High frame-rate resolution of cell division during Candida albicans filamentation

Darren D. Thomson; Judith Berman; Alexandra C. Brand

doi:10.1016/j.fgb.2016.02.001

High frame-rate resolution of cell division during Candida albicans filamentation

Darren D. Thomson, Judith Berman, Alexandra C. Brand

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

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Abstract

The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

Original language	English
Pages (from-to)	54-58
Number of pages	5
Journal	Fungal Genetics and Biology
Volume	88
Early online date	4 Feb 2016
DOIs	https://doi.org/10.1016/j.fgb.2016.02.001
Publication status	Published - Mar 2016

Bibliographical note

Acknowledgements:
Strain YMG7139 was supplied by Peter Sudbery, University of Sheffield. DDT was funded by a BBSRC-DTG, BB/F016964/1, at Aberdeen. ACB is funded by a Royal Society URFUF080611 and a MRC NIRG90671. JB was funded by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) REA grant agreement number 303635; by an European Research Council Advanced Award, number 340087, RAPLODAPT, and an award from the Israel Science foundation (340/13).

Keywords

nuclear division
hypha
septum

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10.1016/j.fgb.2016.02.001Licence: CC BY

High frame-rate resolution of cell division during Candida albicans filamentation
Copyright 2016 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Final published version, 1.02 MBLicence: CC BY

Cite this

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title = "High frame-rate resolution of cell division during Candida albicans filamentation",

abstract = "The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.",

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author = "Thomson, {Darren D.} and Judith Berman and Brand, {Alexandra C.}",

note = "Acknowledgements: Strain YMG7139 was supplied by Peter Sudbery, University of Sheffield. DDT was funded by a BBSRC-DTG, BB/F016964/1, at Aberdeen. ACB is funded by a Royal Society URFUF080611 and a MRC NIRG90671. JB was funded by the People Programme (Marie Curie Actions) of the European Union{\textquoteright}s Seventh Framework Programme (FP7/2007-2013) REA grant agreement number 303635; by an European Research Council Advanced Award, number 340087, RAPLODAPT, and an award from the Israel Science foundation (340/13).",

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AU - Brand, Alexandra C.

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N2 - The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

AB - The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.

KW - nuclear division

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KW - septum

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DO - 10.1016/j.fgb.2016.02.001

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SP - 54

EP - 58

JO - Fungal Genetics and Biology

JF - Fungal Genetics and Biology

ER -

High frame-rate resolution of cell division during Candida albicans filamentation

Abstract

Bibliographical note

Keywords

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