The commensal yeast, Candida albicans, is an opportunistic pathogen in humans and forms filaments called hyphae and pseudohyphae, in which cell division requires precise temporal and spatial control to produce mononuclear cell compartments. High-frame-rate live-cell imaging (1frame/min) revealed that nuclear division did not occur across the septal plane. We detected the presence of nucleolar fragments that may be extrachromosomal molecules carrying the ribosomal RNA genes. Cells occasionally maintained multiple nucleoli, suggesting either polyploidy, multiple nuclei and/or aneuploidy of ChrR., while the migration pattern of sister nuclei differed between unbranched and branched hyphae. The presented movie challenges and extends previous concepts of C. albicans cell division.
Strain YMG7139 was supplied by Peter Sudbery, University of Sheffield. DDT was funded by a BBSRC-DTG, BB/F016964/1, at Aberdeen. ACB is funded by a Royal Society URFUF080611 and a MRC NIRG90671. JB was funded by the People Programme (Marie Curie Actions) of the European Union’s Seventh Framework Programme (FP7/2007-2013) REA grant agreement number 303635; by an European Research Council Advanced Award, number 340087, RAPLODAPT, and an award from the Israel Science foundation (340/13).
- nuclear division