High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

Gillian Milne; Louise A. Walker

doi:10.1007/978-1-0716-2417-3_15

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

Gillian Milne
, Louise A. Walker^* (Corresponding Author)

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Chapter

4 Citations (Scopus)

Abstract

Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.

Original language	English
Title of host publication	Candida auris
Subtitle of host publication	Methods and Protocols
Editors	Alexander Lorenz
Publisher	HUMANA PRESS INC
Chapter	15
Pages	189-201
Number of pages	13
Edition	1st
ISBN (Electronic)	978-1-0716-2417-3
ISBN (Print)	978-1-0716-2416-6
DOIs	https://doi.org/10.1007/978-1-0716-2417-3_15
Publication status	Published - 9 Jun 2022

Publication series

Name	Methods in Molecular Biology
Volume	2517
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Bibliographical note

Funding Information:
The authors acknowledge the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance.

Keywords

Freeze substitution
Fungal cell wall
High-pressure freezing
Transmission electron microscopy

Access to Document

10.1007/978-1-0716-2417-3_15Licence: Unspecified

Cite this

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall. / Milne, Gillian; Walker, Louise A. (Corresponding Author).
Candida auris: Methods and Protocols. ed. / Alexander Lorenz. 1st. ed. HUMANA PRESS INC, 2022. p. 189-201 (Methods in Molecular Biology; Vol. 2517).

Research output: Chapter in Book/Report/Conference proceeding › Chapter

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title = "High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall",

abstract = "Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.",

keywords = "Freeze substitution, Fungal cell wall, High-pressure freezing, Transmission electron microscopy",

author = "Gillian Milne and Walker, \{Louise A.\}",

note = "Funding Information: The authors acknowledge the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance. ",

year = "2022",

month = jun,

day = "9",

doi = "10.1007/978-1-0716-2417-3\_15",

language = "English",

isbn = "978-1-0716-2416-6",

series = "Methods in Molecular Biology",

publisher = "HUMANA PRESS INC",

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editor = "Alexander Lorenz",

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TY - CHAP

T1 - High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

AU - Milne, Gillian

AU - Walker, Louise A.

N1 - Funding Information: The authors acknowledge the Microscopy and Histology Core Facility at the University of Aberdeen for expert assistance.

PY - 2022/6/9

Y1 - 2022/6/9

N2 - Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.

AB - Transmission electron microscopy (TEM) is the main technique used to study the ultrastructure of biological samples. Chemical fixation was considered the main method for preserving samples for TEM; however, it is a relatively slow method of fixation and can result in morphological alterations. Cryofixation using high-pressure freezing (HPF) overcomes the limitations of chemical fixation by preserving samples instantly. Here, we describe our HPF methods optimized for visualizing Candida auris at the ultrastructural level.

KW - Freeze substitution

KW - Fungal cell wall

KW - High-pressure freezing

KW - Transmission electron microscopy

UR - https://www.scopus.com/pages/publications/85131772359

U2 - 10.1007/978-1-0716-2417-3_15

DO - 10.1007/978-1-0716-2417-3_15

M3 - Chapter

C2 - 35674955

AN - SCOPUS:85131772359

SN - 978-1-0716-2416-6

T3 - Methods in Molecular Biology

SP - 189

EP - 201

BT - Candida auris

A2 - Lorenz, Alexander

PB - HUMANA PRESS INC

ER -

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

Abstract

Publication series

Bibliographical note

Keywords

Access to Document

Other files and links

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Microscopy and Histology

Cite this

High-Pressure Freezing and Transmission Electron Microscopy to Visualize the Ultrastructure of the C. auris Cell Wall

Abstract

Publication series

Bibliographical note

Keywords

Access to Document

Other files and links

Fingerprint

Equipment

Microscopy and Histology

Cite this