Staining methods involving Giemsa, thionin or azur A were all used to demonstrate the size and distribution of nucleoids within spores and young substrate hyphae of the actinomycete Streptomyces coelicolor A3(2). The significance of observations made upon nucleoids visualised using such histological strains is questionable, because fixation and other preparative steps alter their state in vivo. The appearance of nucleoids in unfixed preparations following fluorescent staining with DAPI, mithramycin, ethidium bromide, Hoechst-33258 or beberine sulphate was similar and more accurately represented the true disposition of nucleoids within hyphae. Flourescent staining of chemically fixed preparations gave results similar to those of histologically stained material of the same age, that were also subject to fixation procedures. Staining young germ tube hyphae with DAPI also revealed the presence of polyphosphate storage granules.
|Number of pages||10|
|Journal||Journal of Microbiological Methods|
|Publication status||Published - Dec 1990|
- NUCLEAR STAINING