Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Andrea Faedo; Jane C Quinn; Patrick Niall Stoney; Jason E Long; Catherine Dye; Massimo Zollo; John L R Rubenstein; David J Price; Alessandro Bulfone

doi:10.1002/dvdy.20152

Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Andrea Faedo
, Jane C Quinn
, Patrick Niall Stoney
, Jason E Long
, Catherine Dye
, Massimo Zollo
, John L R Rubenstein
, David J Price
, Alessandro Bulfone

Medical Sciences

Stem Cell Research Institute, Dibit, H.S. Raffaele, Via Olgettina 58, 20132 Milan, Italy.

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

Original language	English
Pages (from-to)	614-20
Number of pages	7
Journal	Developmental Dynamics
Volume	231
Issue number	3
DOIs	https://doi.org/10.1002/dvdy.20152
Publication status	Published - Nov 2004

Bibliographical note

Keywords

Animals
Body Patterning
DNA, Complementary
Embryonic Development
Eye Proteins
Gene Expression Profiling
Gene Expression Regulation, Developmental
Genome
Homeodomain Proteins
In Situ Hybridization
Mice
Mice, Knockout
Mutation
Oligonucleotide Array Sequence Analysis
Paired Box Transcription Factors
Repressor Proteins
Reverse Transcriptase Polymerase Chain Reaction
Telencephalon
Time Factors
Transcription Factors

Access to Document

10.1002/dvdy.20152

Cite this

@article{befe61722b0847868d66375e1a67c0b5,

title = "Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon",

abstract = "By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.",

keywords = "Animals, Body Patterning, DNA, Complementary, Embryonic Development, Eye Proteins, Gene Expression Profiling, Gene Expression Regulation, Developmental, Genome, Homeodomain Proteins, In Situ Hybridization, Mice, Mice, Knockout, Mutation, Oligonucleotide Array Sequence Analysis, Paired Box Transcription Factors, Repressor Proteins, Reverse Transcriptase Polymerase Chain Reaction, Telencephalon, Time Factors, Transcription Factors",

author = "Andrea Faedo and Quinn, \{Jane C\} and Stoney, \{Patrick Niall\} and Long, \{Jason E\} and Catherine Dye and Massimo Zollo and Rubenstein, \{John L R\} and Price, \{David J\} and Alessandro Bulfone",

year = "2004",

month = nov,

doi = "10.1002/dvdy.20152",

language = "English",

volume = "231",

pages = "614--20",

journal = "Developmental Dynamics",

issn = "1058-8388",

publisher = "Wiley-Liss Inc.",

number = "3",

}

TY - JOUR

T1 - Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

AU - Faedo, Andrea

AU - Quinn, Jane C

AU - Stoney, Patrick Niall

AU - Long, Jason E

AU - Dye, Catherine

AU - Zollo, Massimo

AU - Rubenstein, John L R

AU - Price, David J

AU - Bulfone, Alessandro

PY - 2004/11

Y1 - 2004/11

N2 - By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

AB - By using a custom-made array containing cDNAs preferentially expressed in the mouse embryonic telencephalon (Porteus et al. [1992] Brain Res Mol Brain Res 12:7-22; and Alessandro Bulfone, unpublished data), we studied the gene expression profile of the Dlx1/Dlx2(-/-) subpallium and Pax6(-/-) pallium. We identified a transcript corresponding to Unigene Cluster Mm.94021 and rat Evf-1, which is down-regulated in the Dlx1/Dlx2(-/-) subpallium and up-regulated in the Pax6(-/-) pallium. Here, we report the expression pattern of this transcript, designated mouse Evf1 (mEvf1), in the prenatal forebrain of wild-type, Dlx1/Dlx2(-/-) and Pax6(-/-) mice using RNA in situ hybridization and reverse transcriptase-polymerase chain reaction. In the wild-type forebrain mEvf1 expression is restricted to the ventral thalamus, hypothalamus, and subpallial telencephalon (caudal, lateral, and medial ganglionic eminences and septal primordia), whereas it is down-regulated in the Dlx1/Dlx2(-/-) subpallium (mainly in caudal, lateral, and medial ganglionic eminences), and up-regulated in the Pax6(-/-) lateral and ventral pallium at embryonic day 12.5 and in the dorsal, lateral, and ventral pallium at embryonic day 14.5.

KW - Animals

KW - Body Patterning

KW - DNA, Complementary

KW - Embryonic Development

KW - Eye Proteins

KW - Gene Expression Profiling

KW - Gene Expression Regulation, Developmental

KW - Genome

KW - Homeodomain Proteins

KW - In Situ Hybridization

KW - Mice

KW - Mice, Knockout

KW - Mutation

KW - Oligonucleotide Array Sequence Analysis

KW - Paired Box Transcription Factors

KW - Repressor Proteins

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Telencephalon

KW - Time Factors

KW - Transcription Factors

U2 - 10.1002/dvdy.20152

DO - 10.1002/dvdy.20152

M3 - Article

C2 - 15376329

SN - 1058-8388

VL - 231

SP - 614

EP - 620

JO - Developmental Dynamics

JF - Developmental Dynamics

IS - 3

ER -

Identification and characterization of a novel transcript down-regulated in Dlx1/Dlx2 and up-regulated in Pax6 mutant telencephalon

Abstract

Bibliographical note

Keywords

Access to Document

Fingerprint

Cite this