Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein

J G Lindh; Bernadette Connolly; D L McGhie; D F Smith

doi:10.1016/S0166-6851(97)00242-9

Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein

J G Lindh, Bernadette Connolly, D L McGhie, D F Smith

Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

Development of the infective L1 larva of Trichinella spiralis occurs as an intracellular parasite of skeletal muscle and leads to the dedifferentiation of the host cell. A novel Trichinella gene, tsJ5, has been identified from a cDNA library screen for sequences encoding Trichinella proteins related to the myogenic bHLH factors. The tsJ5 gene is developmentally regulated, showing preferential expression in the infective muscle stage larva. The product of the tsJ5 gene is not a bHLH protein but represents a novel protein with properties in common with some myogenic repressors. A recombinant TsJ5 protein affects the formation of MyoD:DNA complexes in vitro.

Original language	English
Pages (from-to)	163-175
Number of pages	13
Journal	Molecular and Biochemical Parasitology
Volume	92
Issue number	1
DOIs	https://doi.org/10.1016/S0166-6851(97)00242-9
Publication status	Published - 1 Apr 1998

Keywords

Amino Acid Sequence
Animals
Base Sequence
DNA, Complementary
Gene Expression Regulation
Gene Library
Genes, Helminth
Helminth Proteins
Larva
Molecular Sequence Data
Muscle Proteins
MyoD Protein
Protein Binding
Repressor Proteins
Sequence Homology, Amino Acid
Trichinella spiralis
Trichinella
Infective stage larva
TsJ5
Inhibitor of MyoD
Developmental regulation

Access to Document

10.1016/S0166-6851(97)00242-9

Cite this

@article{84bcccb77d5f4347aacb918b22830435,

title = "Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein",

abstract = "Development of the infective L1 larva of Trichinella spiralis occurs as an intracellular parasite of skeletal muscle and leads to the dedifferentiation of the host cell. A novel Trichinella gene, tsJ5, has been identified from a cDNA library screen for sequences encoding Trichinella proteins related to the myogenic bHLH factors. The tsJ5 gene is developmentally regulated, showing preferential expression in the infective muscle stage larva. The product of the tsJ5 gene is not a bHLH protein but represents a novel protein with properties in common with some myogenic repressors. A recombinant TsJ5 protein affects the formation of MyoD:DNA complexes in vitro.",

keywords = "Amino Acid Sequence, Animals, Base Sequence, DNA, Complementary, Gene Expression Regulation, Gene Library, Genes, Helminth, Helminth Proteins, Larva, Molecular Sequence Data, Muscle Proteins, MyoD Protein, Protein Binding, Repressor Proteins, Sequence Homology, Amino Acid, Trichinella spiralis, Trichinella, Infective stage larva, TsJ5, Inhibitor of MyoD, Developmental regulation",

author = "Lindh, {J G} and Bernadette Connolly and McGhie, {D L} and Smith, {D F}",

year = "1998",

month = apr,

day = "1",

doi = "10.1016/S0166-6851(97)00242-9",

language = "English",

volume = "92",

pages = "163--175",

journal = "Molecular and Biochemical Parasitology",

issn = "0166-6851",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein

AU - Lindh, J G

AU - Connolly, Bernadette

AU - McGhie, D L

AU - Smith, D F

PY - 1998/4/1

Y1 - 1998/4/1

N2 - Development of the infective L1 larva of Trichinella spiralis occurs as an intracellular parasite of skeletal muscle and leads to the dedifferentiation of the host cell. A novel Trichinella gene, tsJ5, has been identified from a cDNA library screen for sequences encoding Trichinella proteins related to the myogenic bHLH factors. The tsJ5 gene is developmentally regulated, showing preferential expression in the infective muscle stage larva. The product of the tsJ5 gene is not a bHLH protein but represents a novel protein with properties in common with some myogenic repressors. A recombinant TsJ5 protein affects the formation of MyoD:DNA complexes in vitro.

AB - Development of the infective L1 larva of Trichinella spiralis occurs as an intracellular parasite of skeletal muscle and leads to the dedifferentiation of the host cell. A novel Trichinella gene, tsJ5, has been identified from a cDNA library screen for sequences encoding Trichinella proteins related to the myogenic bHLH factors. The tsJ5 gene is developmentally regulated, showing preferential expression in the infective muscle stage larva. The product of the tsJ5 gene is not a bHLH protein but represents a novel protein with properties in common with some myogenic repressors. A recombinant TsJ5 protein affects the formation of MyoD:DNA complexes in vitro.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - DNA, Complementary

KW - Gene Expression Regulation

KW - Gene Library

KW - Genes, Helminth

KW - Helminth Proteins

KW - Larva

KW - Molecular Sequence Data

KW - Muscle Proteins

KW - MyoD Protein

KW - Protein Binding

KW - Repressor Proteins

KW - Sequence Homology, Amino Acid

KW - Trichinella spiralis

KW - Trichinella

KW - Infective stage larva

KW - TsJ5

KW - Inhibitor of MyoD

KW - Developmental regulation

U2 - 10.1016/S0166-6851(97)00242-9

DO - 10.1016/S0166-6851(97)00242-9

M3 - Article

C2 - 9574919

SN - 0166-6851

VL - 92

SP - 163

EP - 175

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 1

ER -

Identification of a developmentally regulated Trichinella spiralis protein that inhibits MyoD-specific protein

Abstract

Keywords

Access to Document

Fingerprint

Cite this