Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq

E Malandrakis; O Dadali; M Kavouras; T Danis; P Panagiotaki; H Miliou; F C Kuepper; A Exadactylos

doi:10.1016/j.margen.2017.03.005

Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq

E Malandrakis, O Dadali, M Kavouras, T Danis, P Panagiotaki, H Miliou, F C Kuepper, A Exadactylos

Research output: Contribution to journal › Article › peer-review

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Abstract

Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.

Original language	English
Pages (from-to)	47-56
Number of pages	10
Journal	Marine Genomics
Volume	34
Early online date	4 Apr 2017
DOIs	https://doi.org/10.1016/j.margen.2017.03.005
Publication status	Published - Aug 2017

Bibliographical note

This research has been co-financed by the European Union (European Social Fund – ESF) (375425) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: THALES - Investing in knowledge society through the European Social Funds (Project Acronym: MANTOLES). Also useful suggestions by Professor C. Katsaros are greatly appreciated. The authors would like to thank the two anonymous referees for their essential suggestions and comments. The MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged.

Keywords

Journal Article
Cymodocea nodosa
RNAseq
Transcriptome
Differential expression

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Accepted Manuscript
© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
Accepted author manuscript, 188 KBLicence: CC BY-NC-ND

Cite this

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title = "Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq",

abstract = "Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.",

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author = "E Malandrakis and O Dadali and M Kavouras and T Danis and P Panagiotaki and H Miliou and Kuepper, {F C} and A Exadactylos",

note = "This research has been co-financed by the European Union (European Social Fund – ESF) (375425) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: THALES - Investing in knowledge society through the European Social Funds (Project Acronym: MANTOLES). Also useful suggestions by Professor C. Katsaros are greatly appreciated. The authors would like to thank the two anonymous referees for their essential suggestions and comments. The MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged.",

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journal = "Marine Genomics",

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AU - Malandrakis, E

AU - Dadali, O

AU - Kavouras, M

AU - Danis, T

AU - Panagiotaki, P

AU - Miliou, H

AU - Kuepper, F C

AU - Exadactylos, A

N1 - This research has been co-financed by the European Union (European Social Fund – ESF) (375425) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF) - Research Funding Program: THALES - Investing in knowledge society through the European Social Funds (Project Acronym: MANTOLES). Also useful suggestions by Professor C. Katsaros are greatly appreciated. The authors would like to thank the two anonymous referees for their essential suggestions and comments. The MASTS pooling initiative (Marine Alliance for Science and Technology for Scotland, funded by the Scottish Funding Council and contributing institutions; grant reference HR09011) is gratefully acknowledged.

PY - 2017/8

Y1 - 2017/8

N2 - Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.

AB - Seagrasses exhibit vital ecological roles in the marine environment. Nevertheless, the genomic resources available for seagrasses are still scarce. In the present study, the transcriptome of Cymodocea nodosa was sequenced with a view to study the molecular mechanisms underlying abiotic stress responses. The sequenced transcriptome for the species was near-complete and a high percentage of the transcripts was computationally annotated. An experimental simulation of marine plant exposure to extreme temperature (34°C), salinity (50psu) and their combination was conducted. A dynamic transcriptome 24h response (short-term) from stress initialization was recorded. The most noteworthy alteration in gene expression was observed in heat-stressed plants. Transcripts associated with development, photosynthesis, osmotic balance and stress-response were differentially expressed, under the set experimental conditions. Results indicate a potential negative interaction of heat and osmotic stress on seagrasses transcriptome.

KW - Journal Article

KW - Cymodocea nodosa

KW - RNAseq

KW - Transcriptome

KW - Differential expression

U2 - 10.1016/j.margen.2017.03.005

DO - 10.1016/j.margen.2017.03.005

M3 - Article

C2 - 28389176

SN - 1874-7787

VL - 34

SP - 47

EP - 56

JO - Marine Genomics

JF - Marine Genomics

ER -

Identification of the abiotic stress-related transcription in little Neptune grass Cymodocea nodosa with RNA-seq

Abstract

Bibliographical note

Keywords

UN SDGs

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