Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Andrew Aitken; P Cohen; S Santikarn; Dudley H Williams; Alexander Graham Calder; Alistair Smith; C B Klee

doi:10.1016/0014-5793(82)80759-X

Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Andrew Aitken
, P Cohen
, S Santikarn
, Dudley H Williams
, Alexander Graham Calder
, Alistair Smith
, C B Klee

Medical Sciences

Research output: Contribution to journal › Article › peer-review

210 Citations (Scopus)

Abstract

The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

Original language	English
Pages (from-to)	314-318
Number of pages	5
Journal	FEBS Letters
Volume	150
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(82)80759-X
Publication status	Published - 27 Dec 1982

Keywords

Amino Acids
Animals
Brain Chemistry
Calcium-Binding Proteins
Calmodulin-Binding Proteins
Cattle
Chromatography, Gas
Mass Spectrometry
Myristic Acid
Myristic Acids
Nerve Tissue Proteins
Protein phosphatase
Ca2+
Calmodulin
Mass spectrometry
Fatty acids
High-performance liquid High-performance liquid chromatography

Access to Document

10.1016/0014-5793(82)80759-X

Cite this

@article{8c1d68e4a1524b4f872d67866b5d61d6,

title = "Identification of the NH2-terminal blocking group of calcineurin B as myristic acid",

abstract = "The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57\% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.",

keywords = "Amino Acids, Animals, Brain Chemistry, Calcium-Binding Proteins, Calmodulin-Binding Proteins, Cattle, Chromatography, Gas, Mass Spectrometry, Myristic Acid, Myristic Acids, Nerve Tissue Proteins, Protein phosphatase, Ca2+, Calmodulin, Mass spectrometry, Fatty acids, High-performance liquid High-performance liquid chromatography ",

author = "Andrew Aitken and P Cohen and S Santikarn and Williams, \{Dudley H\} and Calder, \{Alexander Graham\} and Alistair Smith and Klee, \{C B\}",

year = "1982",

month = dec,

day = "27",

doi = "10.1016/0014-5793(82)80759-X",

language = "English",

volume = "150",

pages = "314--318",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley and Sons Inc.",

number = "2",

}

TY - JOUR

T1 - Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

AU - Aitken, Andrew

AU - Cohen, P

AU - Santikarn, S

AU - Williams, Dudley H

AU - Calder, Alexander Graham

AU - Smith, Alistair

AU - Klee, C B

PY - 1982/12/27

Y1 - 1982/12/27

N2 - The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

AB - The NH2-terminal blocking group of the Ca2+-binding B-subunit of calcineurin (protein phosphatase-2B) has been identified as myristic acid by fast atom bombardment mass spectrometry and gas chromatography. The sequence, myristyl-Gly-Asn-Glu-Ala-, is very similar to that of the catalytic subunit of cyclic AMP-dependent protein kinase, the only other protein known to contain this fatty acid. This finding, and the elution of all myristyl peptides at 57% acetonitrile on reverse phase HPLC, may facilitate the identification of other proteins with this blocking group.

KW - Amino Acids

KW - Animals

KW - Brain Chemistry

KW - Calcium-Binding Proteins

KW - Calmodulin-Binding Proteins

KW - Cattle

KW - Chromatography, Gas

KW - Mass Spectrometry

KW - Myristic Acid

KW - Myristic Acids

KW - Nerve Tissue Proteins

KW - Protein phosphatase

KW - Ca2+

KW - Calmodulin

KW - Mass spectrometry

KW - Fatty acids

KW - High-performance liquid High-performance liquid chromatography

U2 - 10.1016/0014-5793(82)80759-X

DO - 10.1016/0014-5793(82)80759-X

M3 - Article

C2 - 7160476

SN - 0014-5793

VL - 150

SP - 314

EP - 318

JO - FEBS Letters

JF - FEBS Letters

IS - 2

ER -

Identification of the NH2-terminal blocking group of calcineurin B as myristic acid

Abstract

Keywords

Access to Document

Fingerprint

Cite this