Identification of vacuole defects in fungi

A. Richards; N.A.R. Gow; V. Veses

doi:10.1016/j.mimet.2012.08.002

Identification of vacuole defects in fungi

A. Richards
, N.A.R. Gow
, V. Veses

Universidad CEU Cardenal Herrera

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

16 Downloads (Pure)

Abstract

Fungal vacuoles are involved in a diverse range of cellular functions, participating in cellular homeostasis, degradation of intracellular components, and storage of ions and molecules. In recent years there has been a significant increase in the number of studies linking these organelles with the regulation of growth and control of cellular morphology, particularly in those fungal species able to undergo yeast-hypha morphogenetic transitions. This has contributed to the refinement of previously published protocols and the development of new techniques, particularly in the area of live-cell imaging of membrane trafficking events and vacuolar dynamics. The current review outlines recent advances in the imaging of fungal vacuoles and assays for characterization of trafficking pathways, and other physiological activities of this important cell organelle.

Original language	English
Pages (from-to)	155-163
Number of pages	9
Journal	Journal of Microbiological Methods
Volume	91
Issue number	1
Early online date	10 Aug 2012
DOIs	https://doi.org/10.1016/j.mimet.2012.08.002
Publication status	Published - Oct 2012

Bibliographical note

Acknowledgements AR was supported by the MRC with a Capacity Building Studentship. NARG thanks the BBSRC and Wellcome Trust for financial support. VV is supported by Universidad CEU Cardenal Herrera (PRCEU14/11 UCH)

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Cite this

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title = "Identification of vacuole defects in fungi",

abstract = "Fungal vacuoles are involved in a diverse range of cellular functions, participating in cellular homeostasis, degradation of intracellular components, and storage of ions and molecules. In recent years there has been a significant increase in the number of studies linking these organelles with the regulation of growth and control of cellular morphology, particularly in those fungal species able to undergo yeast-hypha morphogenetic transitions. This has contributed to the refinement of previously published protocols and the development of new techniques, particularly in the area of live-cell imaging of membrane trafficking events and vacuolar dynamics. The current review outlines recent advances in the imaging of fungal vacuoles and assays for characterization of trafficking pathways, and other physiological activities of this important cell organelle. ",

author = "A. Richards and N.A.R. Gow and V. Veses",

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Identification of vacuole defects in fungi

Abstract

Bibliographical note

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