Immune responses in baboons vaccinated with HIV-2 genetic expression libraries

Christopher P. Locher*, K. F. Sykes, D. J. Blackbourn, S. A. Johnston

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)


Immunization using genetic expression libraries may be an improvement over conventional DNA immunization using a single gene because more epitopes are simultaneously presented to the immune system. In this study, we evaluated the effectiveness of an HIV-2 vaccine made from a genomic expression library in baboons. We found that HIV-2 expression library immunization induced HIV-2-specific memory responses but low levels of CD8+ cell anti-viral responses and neutralizing antibodies. After intravenous virus challenge using a homologous pathogenic variant, HIV-2UC2/9429, viral loads were similar in the HIV-2-immunized and control baboons. We conclude that although immunization using HIV-2 expression libraries induces immune responses, this approach does not provide protection in baboons against intravenous challenge with HIV-2.

Original languageEnglish
Pages (from-to)323-329
Number of pages7
JournalJournal of Medical Primatology
Issue number6
Publication statusPublished - Dec 2002

Bibliographical note

The authors thank Drs. Michelle Leland, Karen Rice, Kenneth D. Carey and the staff at the Southwest Foundation for Biomedical Research for providing veterinary care; Dr. Tran Chanh and Sylvia Trevino for the evaluation of cytotoxic T-cell activity; Drs. Christine Stahl-Henning and Gerhardt Hunsmann of the German Primate Center for providing the recombinant HIV-2BEN vaccinia constructs; Dr. Margery Chaikin of SmithKline Beecham for providing the recombinant HIV-2ST gp120 polypeptide; Dr. Jay Levy and M. S. Mimizeiget of UCSF and Dr. Susan Barnett of the Chiron Corporation for helpful discussions.


  • AIDS
  • DNA vaccine
  • Expression library immunization
  • Gene immunization
  • Papio


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