Increased vimentin in human α- and β-cells in type 2 diabetes

Maaike Roefs, Françoise Carlotti, Katherine Jones, Hannah Wills, Alexander Hamilton, Michael Verschoor, Joanna Williams Durkin, Laura Garcia-Perez, Melissa Brereton, Laura McCulloch, Marten Engelse, Paul Johnson, Barbara Hansen, Kevin Docherty, Eelco de Koning, Anne Clark (Corresponding Author)

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)


Type 2 diabetes (T2DM) is associated with pancreatic islet dysfunction. Loss of β-cell identity has been implicated via dedifferentiation or conversion to other pancreatic endocrine cell types. How these transitions contribute to the onset and progression of T2DM in vivo is unknown. The aims of this study were to determine the degree of epithelial-to-mesenchymal transition occurring in α and β cells in vivo and to relate this to diabetes-associated (patho)physiological conditions. The proportion of islet cells expressing the mesenchymal marker vimentin was determined by immunohistochemistry and quantitative morphometry in specimens of pancreas from human donors with (T2DM, n=28) and without diabetes (ND, n=38), and in non-human primates at different stages of the diabetic syndrome; normoglycaemic (ND, n=4), obese, hyperinsulinaemic (HI, n=4) and hyperglycaemic (DM, n=8). Vimentin co-localised more frequently with glucagon (α-cells) than with insulin (β-cells) in the human ND group (1.43% total α-cells, 0.98% total β-cells, median; p<0.05); these proportions were higher in T2DM than ND (median 4.53% α- , 2.53% β-cells; p<0.05). Vimentin-positive β-cells were not apoptotic, had reduced expression of Nkx6.1 and Pdx-1 and were not associated with islet amyloidosis or with bihormonal expression (insulin+glucagon). In non-human primates, vimentin-positive β-cell proportion was larger in the diabetic than the ND group (12.08% vs. 0.45%, respectively, p<0.05), whereas it was similar in ND and HI groups. In conclusion, islet cell expression of vimentin indicates a degree of plasticity and dedifferentiation with potential loss of cellular identity in diabetes. This could contribute to α- and β-cell dysfunction in T2DM.

Original languageEnglish
Pages (from-to)217-227
Number of pages11
JournalJournal of Endocrinology
Issue number3
Early online date27 Mar 2017
Publication statusPublished - 1 Jun 2017


  • Amyloid
  • differentiation
  • EMT
  • non-human primate
  • islet
  • glucagon
  • insulin


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