Inhibitory effect of Pax4 on the human insulin and islet amyloid polypeptide (IAPP) promoters

S C Campbell, H Cragg, L J Elrick, W M Macfarlane, K I J Shennan, K Docherty

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Pax4 is a paired-box transcription factor that plays an important role in the development of pancreatic beta-cells. Two Pax4 cDNAs were isolated from a rat insulinoma library. One contained the full-length sequence of Pax4, The other, termed Pax4c, was identical to Pax4 but lacked the sequences encoding 117 amino acids at the COOH-terminus, Pax4 was found to inhibit the human insulin promoter through a sequence element, the C2 box, located at -253 to -244, and the islet amyloid poly peptide promoter through a sequence element located downstream of -138, The inhibitory activity of Pax4 was mapped to separate regions of the protein between amino acids 2-230 and 231-349. (C) 1999 Federation of European Biochemical Societies.

Original languageEnglish
Pages (from-to)53-57
Number of pages5
JournalFEBS Letters
Volume463
Publication statusPublished - 1999

Keywords

  • insulin gene
  • diabetes mellitus
  • amylin
  • transcription
  • BETA-CELL LINE
  • MOLECULAR-CLONING
  • MAMMALIAN-CELLS
  • PAIRED DOMAIN
  • GENE
  • TRANSCRIPTION
  • IDENTIFICATION
  • ACTIVATION
  • SEQUENCE
  • PROTEIN

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