Intracellular Ca2+ stores are essential for injury induced Ca2+ signaling and re-endothelialization

Zhiqiang Zhao, Petr Walczysko, Min Zhao

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)


Endothelialization repairs the lining of damaged vasculature and is a key process in preventing thrombosis and restenosis. It has been demonstrated that extracellular calcium ([Ca2+](o)) influx is important for subsequent endothelialization. The role of intracellular Ca2+ stores in mechanical denudation induced intracellular calcium ([Ca2+](i)) rise and endothelialization remains to be demonstrated. Using monolayer culture of a human endothelial cell line (human umbilical vein endothelial cell, HUVEC), we investigated [Ca2+, wave propagation and re-endothelialization following mechanical denudation. Consistent with previous reports for other types of cells, mechanical denudation induces calcium influx, which is essential for [Ca2+](i) rise and endothelialization. Moreover, we found that intracellular Ca2+ stores are also essential for denudation induced[Ca2+](i) wave initiation and propagation, and the subsequent endothelialization. Thapsigargin which depletes intracellular Ca2+ stores completely abolished [Ca2+](i) wave generation and endothelialization. Xestospongin C (XeC), which prevents Ca2+ release from intracellular Ca2+ stores by inhibition of inositol 1,4,5-trisphosphate (IP3) receptor, inhibited intercellular Ca2+ wave generation and endothelialization following denudation. Purinergic signaling through a suramin sensitive mechanism and gap junction communication also contribute to in intercellular Ca2+ wave propagation and re-endothelialization. We conclude that intracellular Ca2+ stores, in addition to extracellular Ca2+, are essential for intracellular Ca2+ signaling and subsequent endothelialization following mechanical denudation.

Original languageEnglish
Pages (from-to)595-603
Number of pages9
JournalJournal of Cellular Physiology
Issue number3
Early online date16 Oct 2007
Publication statusPublished - Mar 2008


  • Calcium Channel Blockers
  • Calcium Channels, L-Type
  • Calcium Signaling
  • Cobalt
  • Endothelial Cells
  • Gap Junctions
  • Humans
  • Receptors, Purinergic


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