Local origin of cells in FGF-4 - induced outgrowth of amputated chick wing bud stumps

K Kostakopoulou, Neil Vargesson, Jonathan D Clarke, P M Brickell, C Tickle

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


Urodele amphibians are the only vertebrates that can regenerate amputated limbs, even as adults. However, we have previously shown that amputated chick wing bud stumps can be induced to ((regenerate)) and to form a complete set of correctly-patterned skeletal elements, following implantation of beads soaked in fibroblast growth factor-4 (FGF-4). We have now performed Dil injection experiments to determine which cells contribute to FGF-4-induced chick wing bud ((regenerates)). We show that the FGF-4-induced outgrowth of the regenerating wing bud stump is comprised of mesenchyme cells that originate from a region within 200 microm of the FGF-4 bead, and that cells proximal to the bead move distally.
Original languageEnglish
Pages (from-to)747-750
Number of pages4
JournalInternational Journal of Developmental Biology
Issue number5
Publication statusPublished - 1 Oct 1997


  • Animals
  • Carbocyanines
  • Chick Embryo
  • Fibroblast Growth Factor 4
  • Fibroblast Growth Factors
  • Fluorescent Dyes
  • Limb Buds
  • Mesoderm
  • Microinjections
  • Proto-Oncogene Proteins
  • Regeneration
  • Wing
  • limb bud regeneration
  • fibroblast growth factor-4
  • DiI injection


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