LUMINESCENCE-BASED DETECTION OF ERWINIA-CAROTOVORA ASSOCIATED WITH ROTTING POTATO-TUBERS

K MCLENNAN, Lesley Anne Glover, Kenneth Stuart Killham, James Ivor Prosser

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

A luminescence-based marker system has been used to follow rotting of potato tubers by Erwinia carotovora. The early stages of infection could be detected by visual observation of areas of luminescence developing from the point of inoculation. During later stages luminescence was limited to peripheral regions and to central regions where the structure of the potato was destroyed. Luminometry demonstrated reduction in aerobic activity of infecting cells due to oxygen limitation. The system enables rapid and sensitive detection of active marked populations and distinction between aerobic and fermentative activity.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalLetters in Applied Microbiology
Volume15
Issue number4
Publication statusPublished - Oct 1992

Keywords

  • PATHOGENESIS
  • SOIL

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