Abstract
Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.
| Original language | English |
|---|---|
| Title of host publication | Bioremediation of Nitroaromatic and Haloaromatic Compounds |
| Editors | Bruce C. Alleman, Andrea Leeson |
| Place of Publication | Columbus |
| Publisher | Battelle Press |
| Pages | 247-252 |
| Number of pages | 6 |
| ISBN (Print) | 1-57477-080-2, 9781574770803 |
| Publication status | Published - 1999 |
| Event | 5th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, Canada Duration: 19 Apr 1999 → 22 Apr 1999 |
Conference
| Conference | 5th International In Situ and On-Site Bioremediation Symposium |
|---|---|
| Country/Territory | Canada |
| City | SAN DIEGO |
| Period | 19/04/99 → 22/04/99 |