Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil

K  Killham; L J  Shaw; A A  Meharg; T  Boucard; L A  Glover; Y  Beaton

Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil

K Killham
, L J Shaw
, A A Meharg
, T Boucard
, L A Glover
, Y Beaton

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

Abstract

Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.

Original language	English
Title of host publication	Bioremediation of Nitroaromatic and Haloaromatic Compounds
Editors	Bruce C. Alleman, Andrea Leeson
Place of Publication	Columbus
Publisher	Battelle Press
Pages	247-252
Number of pages	6
ISBN (Print)	1-57477-080-2, 9781574770803
Publication status	Published - 1999
Event	5th International In Situ and On-Site Bioremediation Symposium - SAN DIEGO, Canada Duration: 19 Apr 1999 → 22 Apr 1999

Conference

Conference	5th International In Situ and On-Site Bioremediation Symposium
Country/Territory	Canada
City	SAN DIEGO
Period	19/04/99 → 22/04/99

Cite this

Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil. / Killham, K ; Shaw, L J ; Meharg, A A et al.
Bioremediation of Nitroaromatic and Haloaromatic Compounds. ed. / Bruce C. Alleman; Andrea Leeson. Columbus: Battelle Press, 1999. p. 247-252.

Research output: Chapter in Book/Report/Conference proceeding › Published conference contribution

Killham, K, Shaw, LJ, Meharg, AA, Boucard, T, Glover, LA & Beaton, Y 1999, Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil. in BC Alleman & A Leeson (eds), Bioremediation of Nitroaromatic and Haloaromatic Compounds. Battelle Press, Columbus, pp. 247-252, 5th International In Situ and On-Site Bioremediation Symposium, SAN DIEGO, Canada, 19/04/99.

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title = "Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil",

abstract = "Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.",

author = "K Killham and Shaw, \{L J\} and Meharg, \{A A\} and T Boucard and Glover, \{L A\} and Y Beaton",

year = "1999",

language = "English",

isbn = "1-57477-080-2",

pages = "247--252",

editor = "Alleman, \{Bruce C.\} and Andrea Leeson",

booktitle = "Bioremediation of Nitroaromatic and Haloaromatic Compounds",

publisher = "Battelle Press",

note = "5th International In Situ and On-Site Bioremediation Symposium ; Conference date: 19-04-1999 Through 22-04-1999",

}

TY - GEN

T1 - Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil

AU - Killham, K

AU - Shaw, L J

AU - Meharg, A A

AU - Boucard, T

AU - Glover, L A

AU - Beaton, Y

PY - 1999

Y1 - 1999

N2 - Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.

AB - Laboratory-based experiments were carried out to establish feasibility of lux-marked biosensors for assessing in-situ bioremediation potential of 2,4-DCP contaminated soil. Results showed that the decreases in 2,4-DCP concentration in soil water extracts from soil inoculated with a 2,4-DCP degrader, corresponded directly to decreases in toxicity to lux-marked E. coli. Response of the biosensor was, therefore, intimately linked to mineralisation activity in inoculated soil. In addition, light output of P. putida 12708 luxCDABE correlated with 2,4-DCP concentration in spiked sterile soil during ageing and in a field contaminated soil undergoing remediation. Lux-modified bacteria may be used as rapid, sensitive and reliable reporters of 2,4-DCP bioremediation and ageing in soil systems, complementing chemical analyses.

M3 - Published conference contribution

SN - 1-57477-080-2

SN - 9781574770803

SP - 247

EP - 252

BT - Bioremediation of Nitroaromatic and Haloaromatic Compounds

A2 - Alleman, Bruce C.

A2 - Leeson, Andrea

PB - Battelle Press

CY - Columbus

T2 - 5th International In Situ and On-Site Bioremediation Symposium

Y2 - 19 April 1999 through 22 April 1999

ER -

Lux-based biosensing of 2,4-dichlorophenol biodegradation and bioavailability in soil

Abstract

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