Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: (CD3)-C-13-L-methionine as a source of the methyl group

P  Andrewes; W R  Cullen; J  Feldmann; I  Koch; E  Polishchuk

Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: (CD3)-C-13-L-methionine as a source of the methyl group

P Andrewes, W R Cullen, J Feldmann, I Koch, E Polishchuk

Chemistry

Research output: Contribution to journal › Article

28 Citations (Scopus)

Abstract

The filamentous fungus Scopulariopsis brevicaulis produces nonvolatile methylantimony compounds (found in the medium) when grown in antimony(III)-rich medium. To investigate the methyl source, (CD3)-C-13-labelled L-methionine was added to the growth medium. After one month sodium borohydride reduction of media samples produced dimethylstibine and trimethylstibine. The methylstibines were separated on a packed GC column and obtained as gaseous fractions. Analysis of the methylstibines, in the gaseous fractions, by CGC-MS (lion-trap) established (CD3)-C-13 incorporation in both the trimethyl-and dimethyl-antimony compounds. Copyright (C) 1999 John Wiley & Sons, Ltd.

Original language	English
Pages (from-to)	681-687
Number of pages	7
Journal	Applied Organometallic Chemistry
Volume	13
Publication status	Published - 1999

Keywords

Scopulariopsis brevicaulis
L-methionine
potassium antimony tartrate
biomethylation
biotransformation
S-adenosylmethionine
methylantimony species
CHROMATOGRAPHY MASS-SPECTROMETRY
APIOTRICHUM-HUMICOLA
ANTIMONY COMPOUNDS
BIOMETHYLATION
SPECIATION

Cite this

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title = "Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: (CD3)-C-13-L-methionine as a source of the methyl group",

abstract = "The filamentous fungus Scopulariopsis brevicaulis produces nonvolatile methylantimony compounds (found in the medium) when grown in antimony(III)-rich medium. To investigate the methyl source, (CD3)-C-13-labelled L-methionine was added to the growth medium. After one month sodium borohydride reduction of media samples produced dimethylstibine and trimethylstibine. The methylstibines were separated on a packed GC column and obtained as gaseous fractions. Analysis of the methylstibines, in the gaseous fractions, by CGC-MS (lion-trap) established (CD3)-C-13 incorporation in both the trimethyl-and dimethyl-antimony compounds. Copyright (C) 1999 John Wiley & Sons, Ltd.",

keywords = "Scopulariopsis brevicaulis, L-methionine, potassium antimony tartrate, biomethylation, biotransformation, S-adenosylmethionine, methylantimony species, CHROMATOGRAPHY MASS-SPECTROMETRY, APIOTRICHUM-HUMICOLA, ANTIMONY COMPOUNDS, BIOMETHYLATION, SPECIATION",

author = "P Andrewes and Cullen, {W R} and J Feldmann and I Koch and E Polishchuk",

year = "1999",

language = "English",

volume = "13",

pages = "681--687",

journal = "Applied Organometallic Chemistry",

issn = "0268-2605",

publisher = "John Wiley and Sons Ltd",

}

TY - JOUR

T1 - Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: (CD3)-C-13-L-methionine as a source of the methyl group

AU - Andrewes, P

AU - Cullen, W R

AU - Feldmann, J

AU - Koch, I

AU - Polishchuk, E

PY - 1999

Y1 - 1999

N2 - The filamentous fungus Scopulariopsis brevicaulis produces nonvolatile methylantimony compounds (found in the medium) when grown in antimony(III)-rich medium. To investigate the methyl source, (CD3)-C-13-labelled L-methionine was added to the growth medium. After one month sodium borohydride reduction of media samples produced dimethylstibine and trimethylstibine. The methylstibines were separated on a packed GC column and obtained as gaseous fractions. Analysis of the methylstibines, in the gaseous fractions, by CGC-MS (lion-trap) established (CD3)-C-13 incorporation in both the trimethyl-and dimethyl-antimony compounds. Copyright (C) 1999 John Wiley & Sons, Ltd.

AB - The filamentous fungus Scopulariopsis brevicaulis produces nonvolatile methylantimony compounds (found in the medium) when grown in antimony(III)-rich medium. To investigate the methyl source, (CD3)-C-13-labelled L-methionine was added to the growth medium. After one month sodium borohydride reduction of media samples produced dimethylstibine and trimethylstibine. The methylstibines were separated on a packed GC column and obtained as gaseous fractions. Analysis of the methylstibines, in the gaseous fractions, by CGC-MS (lion-trap) established (CD3)-C-13 incorporation in both the trimethyl-and dimethyl-antimony compounds. Copyright (C) 1999 John Wiley & Sons, Ltd.

KW - Scopulariopsis brevicaulis

KW - L-methionine

KW - potassium antimony tartrate

KW - biomethylation

KW - biotransformation

KW - S-adenosylmethionine

KW - methylantimony species

KW - CHROMATOGRAPHY MASS-SPECTROMETRY

KW - APIOTRICHUM-HUMICOLA

KW - ANTIMONY COMPOUNDS

KW - BIOMETHYLATION

KW - SPECIATION

M3 - Article

SN - 0268-2605

VL - 13

SP - 681

EP - 687

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

ER -

Methylantimony compound formation in the medium of Scopulariopsis brevicaulis cultures: (CD3)-C-13-L-methionine as a source of the methyl group

Abstract

Keywords

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