Moderate-Intensity Combined Training Induces Lipidomic Changes in Individuals with Obesity and Type 2 Diabetes

Renata Garbellini Duft; Ivan Luiz Padilha Bonfante; Susana Alejandra Palma-Duran; Mara Patrícia Traina Chacon-Mikahil; Julian Leether Griffin; Cláudia Regina Cavaglieri

doi:10.1210/clinem/dgae177

Moderate-Intensity Combined Training Induces Lipidomic Changes in Individuals with Obesity and Type 2 Diabetes

Renata Garbellini Duft^* (Corresponding Author)
, Ivan Luiz Padilha Bonfante
, Susana Alejandra Palma-Duran
, Mara Patrícia Traina Chacon-Mikahil
, Julian Leether Griffin
, Cláudia Regina Cavaglieri

^*Corresponding author for this work

The Rowett Institute of Nutrition and Health

Imperial College London
University of Campinas

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

2 Downloads (Pure)

Abstract

CONTEXT: Alterations in the lipid metabolism are linked to metabolic disorders such as insulin resistance (IR), obesity and type 2 diabetes (T2D). Regular exercise, particularly combined training (CT), is a well-known non-pharmacological treatment that combines aerobic (AT) and resistance (RT) training benefits. However, it is unclear whether moderate-intensity exercise without dietary intervention induces changes in lipid metabolism to promote a 'healthy lipidome'.

OBJECTIVE: The study aimed to investigate the effect of 16 weeks of CT on plasma and white adipose tissue in both sexes, middle-aged subjects with normal weight, obesity and T2D using an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) untargeted lipidomics approach.

METHODS: Body composition, maximum oxygen consumption (VO2 max), strength, and biochemical markers were evaluated before and after the control/training period and correlated with lipid changes. CT consisted of 8 to 10 RT exercises, followed by 35 min of AT (45 -70% VO2 max), 3 times a week for 16 weeks.

RESULTS: The CT significantly reduced the levels of saturated and monounsaturated fatty acid side-chains (SFA/MUFA) in sphingolipids, glycerolipids (GL) and glycerophospholipids (GP) as well as reducing fat mass, circumferences and IR. Increased levels of polyunsaturated fatty acids in GPs, and GLs were also observed, along with increased fat-free mass, VO2 max, and strength (all p < 0.05) after training.

CONCLUSION: Our study stated that 16 weeks of moderate-intensity CT remodelled the lipid metabolism in OB, and T2D individuals, even without dietary intervention, establishing a link between exercise-modulated lipid markers and mechanisms that reduce IR and obesity-related comorbidities.

Original language	English
Article number	dgae177
Number of pages	17
Journal	Journal of Clinical Endocrinology and Metabolism
Early online date	15 Mar 2024
DOIs	https://doi.org/10.1210/clinem/dgae177
Publication status	Published - 31 May 2024

Bibliographical note

The authors thank the volunteers for participating in the study.

Data Availability Statement

Original data generated and analyzed during this study are in-
cluded in this published article or in the data repositories listed
in “References.”

Funding

This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP; regular research grants—process: 2016/08751-3; doctorate fellowship process Nos. 2017/11033-8 and 2019/25817-6); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ; Brazil—financing code 303571/2018-7); and the Brazilian Clinical Trials Registry (RBR-62n5qn). The authors thank the volunteers for participating in the study. The authors thank the UK Consortium for MetAbolic Phenotyping (MAP UK, - Medical Research Council (MR/ S010483/1)) for payment of publication fees.

Funders	Funder number
São Paulo State Research Support Foundation	2016/08751-3, 2017/11033-8, 2019/25817-6
National Council for Scientific and Technological Development Brazil	303571/2018-7
Brazilian Clinical Trials Registry	RBR-62n5qn
Medical Research Council	MR/ S010483/1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

exercise,
obesity
diabetes
insulin resistance
lipidomics
mass spectrometry

Access to Document

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Cite this

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title = "Moderate-Intensity Combined Training Induces Lipidomic Changes in Individuals with Obesity and Type 2 Diabetes",

abstract = "CONTEXT: Alterations in the lipid metabolism are linked to metabolic disorders such as insulin resistance (IR), obesity and type 2 diabetes (T2D). Regular exercise, particularly combined training (CT), is a well-known non-pharmacological treatment that combines aerobic (AT) and resistance (RT) training benefits. However, it is unclear whether moderate-intensity exercise without dietary intervention induces changes in lipid metabolism to promote a 'healthy lipidome'.OBJECTIVE: The study aimed to investigate the effect of 16 weeks of CT on plasma and white adipose tissue in both sexes, middle-aged subjects with normal weight, obesity and T2D using an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) untargeted lipidomics approach.METHODS: Body composition, maximum oxygen consumption (VO2 max), strength, and biochemical markers were evaluated before and after the control/training period and correlated with lipid changes. CT consisted of 8 to 10 RT exercises, followed by 35 min of AT (45 -70\% VO2 max), 3 times a week for 16 weeks.RESULTS: The CT significantly reduced the levels of saturated and monounsaturated fatty acid side-chains (SFA/MUFA) in sphingolipids, glycerolipids (GL) and glycerophospholipids (GP) as well as reducing fat mass, circumferences and IR. Increased levels of polyunsaturated fatty acids in GPs, and GLs were also observed, along with increased fat-free mass, VO2 max, and strength (all p < 0.05) after training.CONCLUSION: Our study stated that 16 weeks of moderate-intensity CT remodelled the lipid metabolism in OB, and T2D individuals, even without dietary intervention, establishing a link between exercise-modulated lipid markers and mechanisms that reduce IR and obesity-related comorbidities.",

keywords = "exercise,, obesity, diabetes, insulin resistance, lipidomics, mass spectrometry",

author = "Duft, \{Renata Garbellini\} and Bonfante, \{Ivan Luiz Padilha\} and Palma-Duran, \{Susana Alejandra\} and Chacon-Mikahil, \{Mara Patr{\'i}cia Traina\} and Griffin, \{Julian Leether\} and Cavaglieri, \{Cl{\'a}udia Regina\}",

note = "The authors thank the volunteers for participating in the study.",

year = "2024",

month = may,

day = "31",

doi = "10.1210/clinem/dgae177",

language = "English",

journal = "Journal of Clinical Endocrinology and Metabolism",

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publisher = "Endocrine Society",

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T1 - Moderate-Intensity Combined Training Induces Lipidomic Changes in Individuals with Obesity and Type 2 Diabetes

AU - Duft, Renata Garbellini

AU - Bonfante, Ivan Luiz Padilha

AU - Palma-Duran, Susana Alejandra

AU - Chacon-Mikahil, Mara Patrícia Traina

AU - Griffin, Julian Leether

AU - Cavaglieri, Cláudia Regina

N1 - The authors thank the volunteers for participating in the study.

PY - 2024/5/31

Y1 - 2024/5/31

N2 - CONTEXT: Alterations in the lipid metabolism are linked to metabolic disorders such as insulin resistance (IR), obesity and type 2 diabetes (T2D). Regular exercise, particularly combined training (CT), is a well-known non-pharmacological treatment that combines aerobic (AT) and resistance (RT) training benefits. However, it is unclear whether moderate-intensity exercise without dietary intervention induces changes in lipid metabolism to promote a 'healthy lipidome'.OBJECTIVE: The study aimed to investigate the effect of 16 weeks of CT on plasma and white adipose tissue in both sexes, middle-aged subjects with normal weight, obesity and T2D using an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) untargeted lipidomics approach.METHODS: Body composition, maximum oxygen consumption (VO2 max), strength, and biochemical markers were evaluated before and after the control/training period and correlated with lipid changes. CT consisted of 8 to 10 RT exercises, followed by 35 min of AT (45 -70% VO2 max), 3 times a week for 16 weeks.RESULTS: The CT significantly reduced the levels of saturated and monounsaturated fatty acid side-chains (SFA/MUFA) in sphingolipids, glycerolipids (GL) and glycerophospholipids (GP) as well as reducing fat mass, circumferences and IR. Increased levels of polyunsaturated fatty acids in GPs, and GLs were also observed, along with increased fat-free mass, VO2 max, and strength (all p < 0.05) after training.CONCLUSION: Our study stated that 16 weeks of moderate-intensity CT remodelled the lipid metabolism in OB, and T2D individuals, even without dietary intervention, establishing a link between exercise-modulated lipid markers and mechanisms that reduce IR and obesity-related comorbidities.

AB - CONTEXT: Alterations in the lipid metabolism are linked to metabolic disorders such as insulin resistance (IR), obesity and type 2 diabetes (T2D). Regular exercise, particularly combined training (CT), is a well-known non-pharmacological treatment that combines aerobic (AT) and resistance (RT) training benefits. However, it is unclear whether moderate-intensity exercise without dietary intervention induces changes in lipid metabolism to promote a 'healthy lipidome'.OBJECTIVE: The study aimed to investigate the effect of 16 weeks of CT on plasma and white adipose tissue in both sexes, middle-aged subjects with normal weight, obesity and T2D using an ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS) untargeted lipidomics approach.METHODS: Body composition, maximum oxygen consumption (VO2 max), strength, and biochemical markers were evaluated before and after the control/training period and correlated with lipid changes. CT consisted of 8 to 10 RT exercises, followed by 35 min of AT (45 -70% VO2 max), 3 times a week for 16 weeks.RESULTS: The CT significantly reduced the levels of saturated and monounsaturated fatty acid side-chains (SFA/MUFA) in sphingolipids, glycerolipids (GL) and glycerophospholipids (GP) as well as reducing fat mass, circumferences and IR. Increased levels of polyunsaturated fatty acids in GPs, and GLs were also observed, along with increased fat-free mass, VO2 max, and strength (all p < 0.05) after training.CONCLUSION: Our study stated that 16 weeks of moderate-intensity CT remodelled the lipid metabolism in OB, and T2D individuals, even without dietary intervention, establishing a link between exercise-modulated lipid markers and mechanisms that reduce IR and obesity-related comorbidities.

KW - exercise,

KW - obesity

KW - diabetes

KW - insulin resistance

KW - lipidomics

KW - mass spectrometry

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DO - 10.1210/clinem/dgae177

M3 - Article

C2 - 38488044

SN - 0021-972X

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

M1 - dgae177

ER -

Moderate-Intensity Combined Training Induces Lipidomic Changes in Individuals with Obesity and Type 2 Diabetes

Abstract

Bibliographical note

Data Availability Statement

Funding

UN SDGs

Keywords

Access to Document

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Cite this