NADH Regeneration: A Case Study of Pt-catalyzed NAD+ Reduction with H2

Tony Saba; Jianwei Li; Joseph W.H.  Burnett; Russell Howe; Panagiotis Kechagiopoulos; Xiaodong Wang

doi:10.1021/acscatal.0c04360

NADH Regeneration: A Case Study of Pt-catalyzed NAD+ Reduction with H2

Tony Saba
, Jianwei Li
, Joseph W.H. Burnett
, Russell Howe
, Panagiotis Kechagiopoulos
, Xiaodong Wang^* (Corresponding Author)

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

66 Citations (Scopus)

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Abstract

This study shows the importance of resolving catalytic performance in the regeneration of the reduced form of nicotinamide adenine dinucleotide (NADH) through activity measurements based on NAD+ conversion and the closure of mass balance via by-product quantification. This approach is applied to assess the performance of supported platinum catalysts with varying points of zero charge, utilizing H2 as a reductant. It was found that Pt/SiO2, which exhibits a net negative charge under the reaction conditions, outperforms the neutral Pt/C and positively charged Pt/MgO due to the favorable electrostatic attraction between the catalyst surface and positively charged (+1) nicotinamide ring. NMR spectroscopy identifies side-products formed during NAD+ hydrogenation.

Original language	English
Pages (from-to)	283-289
Number of pages	7
Journal	ACS Catalysis
Volume	11
Issue number	1
Early online date	18 Dec 2020
DOIs	https://doi.org/10.1021/acscatal.0c04360
Publication status	Published - 1 Jan 2021

Bibliographical note

ACKNOWLEDGMENTS:
This work was supported by The Royal Society (ICA\R1\180317).

Keywords

cofactor
supported Pt
conversion
selectivity
mass balance
by-product

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Cite this

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title = "NADH Regeneration: A Case Study of Pt-catalyzed NAD+ Reduction with H2 ",

abstract = "This study shows the importance of resolving catalytic performance in the regeneration of the reduced form of nicotinamide adenine dinucleotide (NADH) through activity measurements based on NAD+ conversion and the closure of mass balance via by-product quantification. This approach is applied to assess the performance of supported platinum catalysts with varying points of zero charge, utilizing H2 as a reductant. It was found that Pt/SiO2, which exhibits a net negative charge under the reaction conditions, outperforms the neutral Pt/C and positively charged Pt/MgO due to the favorable electrostatic attraction between the catalyst surface and positively charged (+1) nicotinamide ring. NMR spectroscopy identifies side-products formed during NAD+ hydrogenation.",

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note = "ACKNOWLEDGMENTS: This work was supported by The Royal Society (ICA\textbackslash{}R1\textbackslash{}180317). ",

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AU - Howe, Russell

AU - Kechagiopoulos, Panagiotis

AU - Wang, Xiaodong

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AB - This study shows the importance of resolving catalytic performance in the regeneration of the reduced form of nicotinamide adenine dinucleotide (NADH) through activity measurements based on NAD+ conversion and the closure of mass balance via by-product quantification. This approach is applied to assess the performance of supported platinum catalysts with varying points of zero charge, utilizing H2 as a reductant. It was found that Pt/SiO2, which exhibits a net negative charge under the reaction conditions, outperforms the neutral Pt/C and positively charged Pt/MgO due to the favorable electrostatic attraction between the catalyst surface and positively charged (+1) nicotinamide ring. NMR spectroscopy identifies side-products formed during NAD+ hydrogenation.

KW - cofactor

KW - supported Pt

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KW - selectivity

KW - mass balance

KW - by-product

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IS - 1

ER -

NADH Regeneration: A Case Study of Pt-catalyzed NAD+ Reduction with H2

Abstract

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Keywords

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