Negative Smad expression and regulation in the developing chick limb

Neil  Vargesson; Ed Laufer

doi:10.1371/journal.pone.0005173

Negative Smad expression and regulation in the developing chick limb

Neil Vargesson, Ed Laufer

Medical Sciences

Research output: Contribution to journal › Article › peer-review

13 Citations (Scopus)

9 Downloads (Pure)

Abstract

The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery. We report the cloning of one Smad6 and two Smad7 (Smad7a and Smad7b) chick homologs and their expression and regulation in the developing limb. Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb. Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

Original language	English
Article number	e5173
Number of pages	8
Journal	PloS ONE
Volume	4
Issue number	4
DOIs	https://doi.org/10.1371/journal.pone.0005173
Publication status	Published - 8 Apr 2009

Keywords

amino acid sequence
animals
bone morphogenetic proteins
chick embryo
cloning, molecular
extremities
gene expression regulation, developmental
molecular sequence data
phylogeny
Smad6 protein
Smad7 protein
development and embryology
molecular genetics
nucleic acids, purines and pyrimidines

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10.1371/journal.pone.0005173

Negative Smad
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abstract = "The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery. We report the cloning of one Smad6 and two Smad7 (Smad7a and Smad7b) chick homologs and their expression and regulation in the developing limb. Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb. Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.",

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AB - The inhibitory or negative Smads, Smad6 and Smad7, block TGFbeta superfamily signals of both the BMP and TGFbeta classes by antagonizing the intracellular signal transduction machinery. We report the cloning of one Smad6 and two Smad7 (Smad7a and Smad7b) chick homologs and their expression and regulation in the developing limb. Smad6 and Smad7a are expressed in dynamic patterns reflecting the domains of BMP gene expression in the limb. Activation and inhibition of the BMP signaling pathway in limb mesenchyme indicates that negative Smad gene expression is regulated, at least in part, by BMP family signals.

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KW - animals

KW - bone morphogenetic proteins

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KW - cloning, molecular

KW - extremities

KW - gene expression regulation, developmental

KW - molecular sequence data

KW - phylogeny

KW - Smad6 protein

KW - Smad7 protein

KW - development and embryology

KW - molecular genetics

KW - nucleic acids, purines and pyrimidines

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VL - 4

JO - PloS ONE

JF - PloS ONE

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M1 - e5173

ER -

Negative Smad expression and regulation in the developing chick limb

Abstract

Keywords

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