Anandamide can be metabolized by cyclooxygenase-2 to produce prostaglandin E-2 (PGE(2)) ethanolamide. The purpose of this study was to investigate the pharmacology of this novel compound. Radioligand binding experiments in membranes from human embryonic kidney cells transfected with PGE(2) receptor subtypes EP1, EP2, EP3, and EP4 revealed that PGE(2) ethanolamide has pK(i) values of 5.61+/-0.1, 6.33+/-0.01, 6.70+/-0.13, and 6.29+/-0.06, respectively, compared with 8.31+/-0.16, 9.03+/-0.04, 9.34+/-0.06, and 9.10+/-0.04 for PGE(2). PGE(2) inhibits electrically evoked contractions of the guinea pig vas deferens (EP3 receptor-mediated), with a pEC(50) value of 9.09+/-0.06, compared with that of 7.38+/-0.09 for PGE(2) ethanolamide. In the guinea pig trachea, 100 nM PGE(2) and 1 muM PGE(2) ethanolamide produced contractions of 51.8+/-10.6 and 38.9+/-5.6% (of the histamine E-max), respectively. The EP1 receptor antagonist SC-51089 (10 muM) prevented the contractions induced by both compounds. In the presence of 10 muM 8-chlorodibenz[b,f][1,4]oxazepine10(11H)-carboxylic acid, 2-[1-oxo-3-(4-pyridinyl) propyl] hydrazide, monohydrochloride (SC-51089), PGE(2) caused a concentration-related relaxation of histamine-induced contractions of this tissue (EP2 receptor-mediated), the pEC(50) value being 8.29+/-0.17 compared with that of 7.11+/-0.18 for PGE(2) ethanolamide. In the rabbit jugular vein, PGE(2) induces relaxation (EP4 receptor-mediated) with a pEC(50) of 9.35+/-0.25, compared with 7.05+/-0.4 for PGE(2) ethanolamide. In dorsal root ganglion neurons in culture, 3 muM PGE(2) ethanolamide evoked an increase in intracellular calcium concentration in 21% of small-diameter capsaicin-sensitive neurons. We conclude that this compound is pharmacologically active, however its physiological relevance has yet to be established.
|Number of pages||7|
|Journal||Journal of Pharmacology and Experimental Therapeutics|
|Publication status||Published - 1 Jun 2002|
- DEPENDENT PROTEIN-KINASE
- ACID AMIDE HYDROLASE
- PROSTANOID RECEPTORS