Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Muhammad Saeed; Pingfang Yang; Ming Li; Umar Masood Quraishi; Ghazala Mustafa; Riffat Naseem Malik

doi:10.1016/j.envexpbot.2024.105812

Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Muhammad Saeed
, Pingfang Yang
, Ming Li
, Umar Masood Quraishi
, Ghazala Mustafa^* (Corresponding Author)
, Riffat Naseem Malik^* (Corresponding Author)

^*Corresponding author for this work

Biological Sciences

Quaid-I-Azam University

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

Arsenic (As) contaminated irrigation water caused a significant buildup of its level in the agricultural soil and edible tissues of wheat. High levels of As negatively impact grain quality, wheat yield, and human health consequently. It is still unknown how wheat varieties respond to As stress at the proteomic level. Therefore, the present study aimed to deliver mechanistic details regarding the role of proteins in tolerance mechanism against As stress. Two wheat varieties (BARANI-70 and NARC-2009) were exposed to arsenate treatment (200 µM) in a hydroponic experiment to evaluate the impacts of arsenic stress using proteomics. Roots of both varieties were used for protein extraction, digestion, and LC-MS/MS analysis to identify differentially abundant proteins (DAPs). Furthermore, various bioinformatic tools were used to study subcellular localization, functional categories, and pathway analysis. The findings identified 67 DAPs in BARANI-70 (up-regulated=56; down-regulated=11) and 89 DAPs in NARC-2009 (up-regulated=34; down-regulated=55). Subcellular localization revealed significant up-regulation of DAPs in mitochondria and chloroplast for BARANI-70 but down-regulation in NARC-2009 under As stress. Functional analysis revealed significant upregulation of DAPs for glutathione, peroxidase, glutathione peroxidase, lactoylglutathione lyase, and glutathione-s-transferase in BARANI-70. Pathway analysis showed regulation of DAPs involved in tetrapyrrole biosynthesis and glycolysis-TCA particularly in BARANI-70 compared to NARC-2009. Furthermore, BARANI-70 showed better growth response under As stress compared to NARC-2009. Thus, better synthesis of stress-responsive DAPs and important pathways in BARANI-70 contributed towards its tolerance against As stress. The present research supports the use of tolerant varieties and identified protein biomarkers to enhance crop resilience against As stress.

Original language	English
Article number	105812
Number of pages	17
Journal	Environmental and Experimental Botany
Volume	224
Early online date	17 May 2024
DOIs	https://doi.org/10.1016/j.envexpbot.2024.105812
Publication status	Published - 1 Aug 2024

Bibliographical note

Acknowledgement
The authors acknowledge the contributions of Junaid Shahzad (Quaid-i-Azam University, Pakistan) for the data analysis in the present study.

Data Availability Statement

The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the iProX partner repository (Chen et al., 2022, Ma et al., 2019) with the dataset identifier PXD046761.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Arsenic-tolerant
Oxidative stress
Mitochondria
Glycolysis
Stress-responsive Proteins

Access to Document

10.1016/j.envexpbot.2024.105812Licence: Unspecified

Data From: Proteomics profiling reveals the detoxification and tolerance behaviour of two bread wheat (Triticum aestivum L.) varieties under arsenate stress
Saeed, M. (Creator), Yang, P. (Creator), Li, M. (Creator), Quraishi, U. M. (Creator), Mustafa, G. (Creator), Malik, R. N. (Creator) & Paton, G. (Other), University of Aberdeen, 2024
DOI: 10.1016/j.envexpbot.2024.105812, https://ars.els-cdn.com/content/image/1-s2.0-S0098847224001709-mmc1.docx and one more link, http://proteomecentral.proteomexchange.org/ (show fewer)
Dataset

Cite this

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title = "Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress",

abstract = "Arsenic (As) contaminated irrigation water caused a significant buildup of its level in the agricultural soil and edible tissues of wheat. High levels of As negatively impact grain quality, wheat yield, and human health consequently. It is still unknown how wheat varieties respond to As stress at the proteomic level. Therefore, the present study aimed to deliver mechanistic details regarding the role of proteins in tolerance mechanism against As stress. Two wheat varieties (BARANI-70 and NARC-2009) were exposed to arsenate treatment (200 µM) in a hydroponic experiment to evaluate the impacts of arsenic stress using proteomics. Roots of both varieties were used for protein extraction, digestion, and LC-MS/MS analysis to identify differentially abundant proteins (DAPs). Furthermore, various bioinformatic tools were used to study subcellular localization, functional categories, and pathway analysis. The findings identified 67 DAPs in BARANI-70 (up-regulated=56; down-regulated=11) and 89 DAPs in NARC-2009 (up-regulated=34; down-regulated=55). Subcellular localization revealed significant up-regulation of DAPs in mitochondria and chloroplast for BARANI-70 but down-regulation in NARC-2009 under As stress. Functional analysis revealed significant upregulation of DAPs for glutathione, peroxidase, glutathione peroxidase, lactoylglutathione lyase, and glutathione-s-transferase in BARANI-70. Pathway analysis showed regulation of DAPs involved in tetrapyrrole biosynthesis and glycolysis-TCA particularly in BARANI-70 compared to NARC-2009. Furthermore, BARANI-70 showed better growth response under As stress compared to NARC-2009. Thus, better synthesis of stress-responsive DAPs and important pathways in BARANI-70 contributed towards its tolerance against As stress. The present research supports the use of tolerant varieties and identified protein biomarkers to enhance crop resilience against As stress.",

keywords = "Arsenic-tolerant, Oxidative stress, Mitochondria, Glycolysis, Stress-responsive Proteins",

author = "Muhammad Saeed and Pingfang Yang and Ming Li and Quraishi, \{Umar Masood\} and Ghazala Mustafa and Malik, \{Riffat Naseem\}",

note = "Acknowledgement The authors acknowledge the contributions of Junaid Shahzad (Quaid-i-Azam University, Pakistan) for the data analysis in the present study.",

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doi = "10.1016/j.envexpbot.2024.105812",

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T1 - Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

AU - Saeed, Muhammad

AU - Yang, Pingfang

AU - Li, Ming

AU - Quraishi, Umar Masood

AU - Mustafa, Ghazala

AU - Malik, Riffat Naseem

N1 - Acknowledgement The authors acknowledge the contributions of Junaid Shahzad (Quaid-i-Azam University, Pakistan) for the data analysis in the present study.

PY - 2024/8/1

Y1 - 2024/8/1

N2 - Arsenic (As) contaminated irrigation water caused a significant buildup of its level in the agricultural soil and edible tissues of wheat. High levels of As negatively impact grain quality, wheat yield, and human health consequently. It is still unknown how wheat varieties respond to As stress at the proteomic level. Therefore, the present study aimed to deliver mechanistic details regarding the role of proteins in tolerance mechanism against As stress. Two wheat varieties (BARANI-70 and NARC-2009) were exposed to arsenate treatment (200 µM) in a hydroponic experiment to evaluate the impacts of arsenic stress using proteomics. Roots of both varieties were used for protein extraction, digestion, and LC-MS/MS analysis to identify differentially abundant proteins (DAPs). Furthermore, various bioinformatic tools were used to study subcellular localization, functional categories, and pathway analysis. The findings identified 67 DAPs in BARANI-70 (up-regulated=56; down-regulated=11) and 89 DAPs in NARC-2009 (up-regulated=34; down-regulated=55). Subcellular localization revealed significant up-regulation of DAPs in mitochondria and chloroplast for BARANI-70 but down-regulation in NARC-2009 under As stress. Functional analysis revealed significant upregulation of DAPs for glutathione, peroxidase, glutathione peroxidase, lactoylglutathione lyase, and glutathione-s-transferase in BARANI-70. Pathway analysis showed regulation of DAPs involved in tetrapyrrole biosynthesis and glycolysis-TCA particularly in BARANI-70 compared to NARC-2009. Furthermore, BARANI-70 showed better growth response under As stress compared to NARC-2009. Thus, better synthesis of stress-responsive DAPs and important pathways in BARANI-70 contributed towards its tolerance against As stress. The present research supports the use of tolerant varieties and identified protein biomarkers to enhance crop resilience against As stress.

AB - Arsenic (As) contaminated irrigation water caused a significant buildup of its level in the agricultural soil and edible tissues of wheat. High levels of As negatively impact grain quality, wheat yield, and human health consequently. It is still unknown how wheat varieties respond to As stress at the proteomic level. Therefore, the present study aimed to deliver mechanistic details regarding the role of proteins in tolerance mechanism against As stress. Two wheat varieties (BARANI-70 and NARC-2009) were exposed to arsenate treatment (200 µM) in a hydroponic experiment to evaluate the impacts of arsenic stress using proteomics. Roots of both varieties were used for protein extraction, digestion, and LC-MS/MS analysis to identify differentially abundant proteins (DAPs). Furthermore, various bioinformatic tools were used to study subcellular localization, functional categories, and pathway analysis. The findings identified 67 DAPs in BARANI-70 (up-regulated=56; down-regulated=11) and 89 DAPs in NARC-2009 (up-regulated=34; down-regulated=55). Subcellular localization revealed significant up-regulation of DAPs in mitochondria and chloroplast for BARANI-70 but down-regulation in NARC-2009 under As stress. Functional analysis revealed significant upregulation of DAPs for glutathione, peroxidase, glutathione peroxidase, lactoylglutathione lyase, and glutathione-s-transferase in BARANI-70. Pathway analysis showed regulation of DAPs involved in tetrapyrrole biosynthesis and glycolysis-TCA particularly in BARANI-70 compared to NARC-2009. Furthermore, BARANI-70 showed better growth response under As stress compared to NARC-2009. Thus, better synthesis of stress-responsive DAPs and important pathways in BARANI-70 contributed towards its tolerance against As stress. The present research supports the use of tolerant varieties and identified protein biomarkers to enhance crop resilience against As stress.

KW - Arsenic-tolerant

KW - Oxidative stress

KW - Mitochondria

KW - Glycolysis

KW - Stress-responsive Proteins

U2 - 10.1016/j.envexpbot.2024.105812

DO - 10.1016/j.envexpbot.2024.105812

M3 - Article

SN - 0098-8472

VL - 224

JO - Environmental and Experimental Botany

JF - Environmental and Experimental Botany

M1 - 105812

ER -

Proteomics profiling reveals the detoxification and tolerance behavior of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Abstract

Bibliographical note

Data Availability Statement

UN SDGs

Keywords

Access to Document

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Datasets

Data From: Proteomics profiling reveals the detoxification and tolerance behaviour of two bread wheat (Triticum aestivum L.) varieties under arsenate stress

Cite this