Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 μg/mL.
This project was supported by grants of the BMBF (to P.P.) and MOST (to W.L.). We wish to thank W.E.G. Müller (Johannes-Gutenberg-University, Mainz, Germany) for carrying out the cytotoxicity assay. A scholarship (Grant No. 10/6/117) granted and financed by the Egyptian Government (Ministry of High Education) to W.E. is gratefully acknowledged.
- Mangrove plants
- Marfey's method
- Structure elucidation