Pullularins E and F, two new peptides from the endophytic fungus Bionectria ochroleuca isolated from the mangrove plant Sonneratia caseolaris

Weaam Ebrahim; Julia Kjer; Mustapha El Amrani; Victor Wray; Wenhan Lin; Rainer Ebel; Daowan Lai; Peter Proksch

doi:10.3390/md10051081

Pullularins E and F, two new peptides from the endophytic fungus Bionectria ochroleuca isolated from the mangrove plant Sonneratia caseolaris

Weaam Ebrahim, Julia Kjer, Mustapha El Amrani, Victor Wray, Wenhan Lin, Rainer Ebel, Daowan Lai^*, Peter Proksch

^*Corresponding author for this work

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Abstract

Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC₅₀ values ranging between 0.1 and 6.7 μg/mL.

Original language	English
Pages (from-to)	1081-1091
Number of pages	11
Journal	Marine Drugs
Volume	10
Issue number	5
Early online date	18 May 2012
DOIs	https://doi.org/10.3390/md10051081
Publication status	Published - 18 May 2012

Bibliographical note

Acknowledgements
This project was supported by grants of the BMBF (to P.P.) and MOST (to W.L.). We wish to thank W.E.G. Müller (Johannes-Gutenberg-University, Mainz, Germany) for carrying out the cytotoxicity assay. A scholarship (Grant No. 10/6/117) granted and financed by the Egyptian Government (Ministry of High Education) to W.E. is gratefully acknowledged.

Keywords

Bionectria
Endophytes
Mangrove plants
Marfey's method
Peptide
Sonneratia
Structure elucidation

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10.3390/md10051081Licence: CC BY

Pullularins E and F, Two New Peptides from the Endophytic Fungus Bionectria ochroleuca Isolated from the Mangrove Plant Sonneratia caseolaris
© 2012 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
Final published version, 236 KBLicence: CC BY

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title = "Pullularins E and F, two new peptides from the endophytic fungus Bionectria ochroleuca isolated from the mangrove plant Sonneratia caseolaris",

abstract = "Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 μg/mL.",

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AU - Ebel, Rainer

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AU - Proksch, Peter

N1 - Acknowledgements This project was supported by grants of the BMBF (to P.P.) and MOST (to W.L.). We wish to thank W.E.G. Müller (Johannes-Gutenberg-University, Mainz, Germany) for carrying out the cytotoxicity assay. A scholarship (Grant No. 10/6/117) granted and financed by the Egyptian Government (Ministry of High Education) to W.E. is gratefully acknowledged.

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AB - Chemical investigation of the EtOAc extract of the endophytic fungus Bionectria ochroleuca, isolated from the inner leaf tissues of the plant Sonneratia caseolaris (Sonneratiaceae) from Hainan island (China), yielded two new peptides, pullularins E and F (1 and 2) together with three known compounds (3-5). The structures of the new compounds were unambiguously determined on the basis of one- and two-dimensional NMR spectroscopy as well as by high-resolution mass spectrometry. The absolute configurations of amino acids were determined by HPLC analysis of acid hydrolysates using Marfey's method. The isolated compounds exhibited pronounced to moderate cytotoxic activity against the mouse lymphoma cells (L5178Y) with EC50 values ranging between 0.1 and 6.7 μg/mL.

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KW - Mangrove plants

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Pullularins E and F, two new peptides from the endophytic fungus Bionectria ochroleuca isolated from the mangrove plant Sonneratia caseolaris

Abstract

Bibliographical note

Keywords

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