Salicylate modulates oxidative stress in the rat colon: a proteomic approach

Janice E. Drew, Sara Padidar, Graham Horgan, Garry G. Duthie, Wendy R. Russell, Martin Reid, Gary Duncan, Garry J. Rucklidge

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)


The dietary phenolic compound, salicylic acid, decreases oxidative stress and pro-inflammatory and potentially neo-plastic prostaglandins with a concomitant increase in glutathione peroxidase activity. Salicylic acid, a dietary plant-based phenolic compound and also the main metabolite of aspirin, may contribute to the colon protective effects of plant-based diets. Oxidative stress is a characteristic of pre-cancerous and cancerous colon and inflammatory bowel diseases (IBD) that increase colon cancer risk. The mechanism(s) whereby salicylic acid modulates potentially pro-cancerous activity associated with oxidative stress is further investigated here using a proteomic approach. A rat model of oxidative stress was supplemented with salicylic acid (1 mg/kg diet, mean plasma levels 310 +/- 32 mu mol/l). Soluble colon protein extracts were subjected to 2D PAGE. Salicylic acid modulated proteins, identified using MALDI-TOF and LC/MS/MS, are involved in protein folding, transport, redox, energy metabolism and cytoskeletal regulation. A partial least squares (PLS) analysis approach was used to assist biological interpretation of the altered protein profiles via their associations between previously published biochemical measurements of oxidative stress, prostaglandin levels and increased glutathione peroxidase activity. Early detection of altered homeostasis in colon may assist in identifying prepathological features preceding colon tumorigenesis and contribute to an understanding of epidemiological evidence supporting a protective effect of plant-based diets. (c) 2006 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)204-216
Number of pages13
JournalBiochemical Pharmacology
Issue number2
Early online date27 Apr 2006
Publication statusPublished - 14 Jul 2006


  • mitochondria
  • redox status
  • phenolic compound
  • partial least squares
  • familial adenomatous polyposis
  • prevent colorectal adenomas
  • protein disulfide-isomerase
  • ulcerative-colitis
  • randomized-trial
  • redox regulation
  • Crohns-disease
  • cancer
  • aspirin
  • thioredoxin


Dive into the research topics of 'Salicylate modulates oxidative stress in the rat colon: a proteomic approach'. Together they form a unique fingerprint.

Cite this