Sialoadhesin promotes rapid proinflammatory and type I IFN responses to a sialylated pathogen, Campylobacter jejuni

Mariliis Klass, Cornelia Oetke, Leanne E. Lewis, Lars P. Erwig, Astrid P. Heikema, Alistair Easton, Hugh J. Willison, Paul R. Crocker

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65 Citations (Scopus)


Sialoadhesin (Sn) is a macrophage (M-phi)-restricted receptor that recognizes sialylated ligands on host cells and pathogens. Although Sn is thought to be important in cellular interactions of M-phis with cells of the immune system, the functional consequences of pathogen engagement by Sn are unclear. As a model system, we have investigated the role of Sn in M-phi interactions with heat-killed Campylobacter jejuni expressing a GD1a-like, sialylated glycan. Compared to Sn-expressing bone marrow-derived macrophages (BMDM) from wild-type mice, BMDM from mice either deficient in Sn or expressing a non-glycan–binding form of Sn showed greatly reduced phagocytosis of sialylated C. jejuni. This was accompanied by a strong reduction in MyD88-dependent secretion of TNF-alpha, IL-6, IL-12, and IL-10. In vivo studies demonstrated that functional Sn was required for rapid TNF-alpha and IFN-beta responses to i.v.-injected sialylated C. jejuni. Bacteria were captured within minutes after i.v. injection and were associated with M-phis in both liver and spleen. In the spleen, IFN-beta–reactive cells were localized to Sn+ M-phis and other cells in the red pulp and marginal zone. Together, these studies demonstrate that Sn plays a key role in capturing sialylated pathogens and promoting rapid proinflammatory cytokine and type I IFN responses.
Original languageEnglish
Pages (from-to)2414-2422
Number of pages9
JournalThe Journal of Immunology
Issue number5
Early online date30 Jul 2012
Publication statusPublished - 1 Sept 2012

Bibliographical note

PMID: 22851711 [PubMed - indexed for MEDLINE] PMCID: PMC3442253 Free PMC Article


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