TY - JOUR
T1 - Sponge-associated fungi and their bioactive compounds
T2 - The Suberites case
AU - Proksch, P.
AU - Ebel, R.
AU - Edrada, R.
AU - Riebe, F.
AU - Liu, H.
AU - Diesel, A.
AU - Bayer, M.
AU - Li, X.
AU - Han Lin, W.
AU - Grebenyuk, V.
AU - Müller, W.E.G.
AU - Draeger, S.
AU - Zuccaro, A.
AU - Schulz, B.
N1 - Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2008/3
Y1 - 2008/3
N2 - Specimens of Suberites domuncula that had been cultured in aquaria for 4 weeks were analyzed for their associated fungi. A total of 81 fungal strains belonging to 20 different genera was isolated and identified by morphological and molecular methods. The most frequently isolated taxa were Cladosporium spp., Penicillium spp., Petriella sp., Phialophora spp. and Engyodontium album. Based on chromatographic and mass spectrometric analysis of fungal extracts, as well as on bioassay results, Aspergillus ustus, Penicillium sp., Petriella sp. and Scopulariopsis sp. were selected for in-depth analysis of their natural products. A total of 19 different fungal metabolites, including three new natural products, was isolated and structurally identified. A. ustus yielded two sesquiterpenes, a drimane derivative and deoxyuvidin, as well as a sesterterpene ophiobolin H. The drimane derivative had an ED value against L5178Y cells of 1.9 µg ml in vitro. The crude extract of Petriella sp. was also strongly cytotoxic against the L5178Y cell line. The cyclic tetrapeptide WF-3161 was primarily responsible for the activity; the ED value was
AB - Specimens of Suberites domuncula that had been cultured in aquaria for 4 weeks were analyzed for their associated fungi. A total of 81 fungal strains belonging to 20 different genera was isolated and identified by morphological and molecular methods. The most frequently isolated taxa were Cladosporium spp., Penicillium spp., Petriella sp., Phialophora spp. and Engyodontium album. Based on chromatographic and mass spectrometric analysis of fungal extracts, as well as on bioassay results, Aspergillus ustus, Penicillium sp., Petriella sp. and Scopulariopsis sp. were selected for in-depth analysis of their natural products. A total of 19 different fungal metabolites, including three new natural products, was isolated and structurally identified. A. ustus yielded two sesquiterpenes, a drimane derivative and deoxyuvidin, as well as a sesterterpene ophiobolin H. The drimane derivative had an ED value against L5178Y cells of 1.9 µg ml in vitro. The crude extract of Petriella sp. was also strongly cytotoxic against the L5178Y cell line. The cyclic tetrapeptide WF-3161 was primarily responsible for the activity; the ED value was
UR - http://www.scopus.com/inward/record.url?scp=45549083173&partnerID=8YFLogxK
U2 - 10.1515/BOT.2008.014
DO - 10.1515/BOT.2008.014
M3 - Article
AN - SCOPUS:45549083173
SN - 0006-8055
VL - 51
SP - 209
EP - 218
JO - Botanica Marina
JF - Botanica Marina
IS - 3
ER -